Nutritional requirements of the BY series of Saccharomyces cerevisiae strains for optimum growth
Nutritional requirements of the BY series of Saccharomyces cerevisiae strains for optimum growth
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DOI：
10.1111/j.1567-1364.2012.00830.x
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发表时间：
2012-11-01
影响因子：
3.2
通讯作者：
Natter, Klaus
中科院分区：
文献类型：
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作者：
Hanscho, Michael;Ruckerbauer, David E.;Natter, Klaus
在种类繁多的酿酒酵母菌株中，BY家族尤为重要，因为广泛使用的缺失收集就是基于这一背景。在这里，我们证明了一些标准的生长介质配方需要进行实质性的修改，以便为菌株的营养缺乏症提供最佳的生长条件，并避免在葡萄糖耗尽之前生长停滞。除了满足营养缺乏症所需的必需补充剂外，我们发现苯丙氨酸、谷氨酸、丝氨酸和苏氨酸这四种氨基酸是最适生长所必需的，尽管这些氨基酸是原养的。有趣的是，其他广泛使用的酿酒酵母菌株，如CEN.PK家族的菌株，对缺乏所描述的补充剂不那么敏感。此外，我们发现酵母氮基中的肌醇浓度太低，不能支持酵母培养物的快速增殖，直到葡萄糖耗尽。在指数生长过程中消耗肌醇会导致特征性的变化，即葡萄糖摄取量和最大比生长率下降，细胞尺寸增加，存活率降低，脂质储存池积累。因此，现有的几种生长介质配方需要修改，以实现副生菌的最佳生长条件。
Among the vast variety of Saccharomyces cerevisiae strains, the BY family is particularly important because the widely used deletion collections are based on this background. Here we demonstrate that some standard growth media recipes require substantial modifications to provide optimum growth conditions for auxotrophic BY strains and to avoid growth arrest before glucose is depleted. In addition to the essential supplements that are required to satisfy auxotrophic requirements, we found the four amino acids phenylalanine, glutamic acid, serine, and threonine to be indispensable for optimum growth, despite the fact that BY is prototrophic for these amino acids. Interestingly, other widely used S. cerevisiae strains, such as strains of the CEN.PK family, are less sensitive to lack of the described supplements. Furthermore, we found that the concentration of inositol in yeast nitrogen base is too low to support fast proliferation of yeast cultures until glucose is exhausted. Depletion of inositol during exponential growth induces characteristic changes, namely a decrease in glucose uptake and maximum specific growth rate, increased cell size, reduced viability, and accumulation of lipid storage pools. Thus, several of the existing growth media recipes need to be revised to achieve optimum growth conditions for BY-derived strains.