Interleukin-10 and Interleukin-10 Receptor-I Are Upregulated in Glial Cells After an Excitotoxic Injury to the Postnatal Rat Brain
Interleukin-10 and Interleukin-10 Receptor-I Are Upregulated in Glial Cells After an Excitotoxic Injury to the Postnatal Rat Brain
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DOI：
10.1097/nen.0b013e31819dca30
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发表时间：
2009-04-01
影响因子：
3.2
通讯作者：
Gonzalez, Berta
中科院分区：
文献类型：
--
作者：
Gonzalez, Pau;Burgaya, Ferran;Gonzalez, Berta
炎症是中枢神经系统损伤严重程度和预后的重要决定因素。内源性抗炎细胞因子白介素-10 （IL-10）在受伤的成人中枢神经系统中上调，在那里它控制和终止炎症过程。然而，发育中的大脑对损伤的易感性和相关的炎症反应与成人大脑不同：未成熟大脑在兴奋性毒性损伤后损伤诱导的IL-10表达的解剖和时间模式尚不清楚。采用定量逆转录酶聚合酶链反应、酶联免疫吸附法分析了9日龄n -甲基-d -天冬氨酸致兴奋性损伤大鼠和对照大鼠中IL-10及其受体（IL-10RI）的时空基因和蛋白表达。和免疫组织化学。我不注射控制大脑。这两种分子主要在胶质细胞和血管上的白质中表达，在灰质和海马、脑膜和脑室表面附近的胶质原纤维酸性蛋白阳性过程中也观察到IL-10。注射n -甲基- d -天冬氨酸的脑组织中，IL-10基因和蛋白表达在注射后72小时达到峰值，IL-10RI基因和蛋白表达在注射后48小时达到峰值。损伤区白细胞介素-10和IL-10RI的表达主要见于反应性星形胶质细胞和小胶质细胞/巨噬细胞。IL-10和IL-10R的表达模式提示可能的发育作用，损伤后IL-10和IL-10R的表达上调提示，，；这种表达可能在未成熟大脑的不同解剖部位具有抗炎作用。
Inflammation is an important determinant of the severity and outcome of central nervous system injury. The endogenous anti-inflammatory cytokine interleukin-10 (IL-10) is upregulated in the injured adult central nervous system where it controls and terminates inflammatory processes. The developing brain, however, displays differences in susceptibility to insults and in associated inflammatory responses from the adult brain: the anatomic and temporal patterns of injury-induced IL-10 expression in the immature brain after excitotoxic injury are unknown. We analyzed the spaciotemporal gene and protein expression of IL-10 and its receptor (IL-10RI) in N-methyl-D-aspartate-induced excitotoxic injury in 9-day-old and control rats using quantitative reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay. and immunohistochemistry. Ill noninjected control brains. both molecules were expressed mainly in white matter on glial cells and blood vessels: IL-10 was also observed oil blood vessels in gray matter and in glial fibrillary acidic protein-positive processes ill the hippocampus and near leptomeningeal and ventricle surfaces. In N-methyl-D-aspartate-injected brains, IL-10 gene and protein expression,were maximal at 72 hours post-injection: IL-10RI gene and protein expression peaked at 48 hours post-injection. Interleukin-10 and IL-10RI expression in injured areas was mainly found in reactive astrocytes and in microglia/macrophages. The expression patterns of IL-10 and IL-10R suggest possible developmental roles, and their upregulation after injury Suggest,,; that this expression may have anti-inflammatory effects in distinct anatomic sites in the immature brain.