The herpes simplex virus receptor nectin-1 is down-regulated after trans-interaction with glycoprotein D.
The herpes simplex virus receptor nectin-1 is down-regulated after trans-interaction with glycoprotein D.
复制标题
单纯疱疹病毒受体 nectin-1 在与糖蛋白 D 反式相互作用后下调。
DOI：
10.1016/j.virol.2007.11.012
复制
发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Krummenacher,Claude
中科院分区：
文献类型：
--
作者：
Stiles,KatieM;Milne,RichardSB;Cohen,GaryH;Eisenberg,RoselynJ;Krummenacher,Claude
在单纯疱疹病毒（HSV）侵入时，膜融合发生在细胞表面或病毒内吞后。在这两种情况下，糖蛋白D （gD）结合到受体如连接素-1或HVEM是必需的。在本研究中，我们将表达gD的细胞与表达nectin-1的细胞共培养，以研究gD对细胞接触时nectin-1的影响。与gD表达细胞共培养过夜后，B78H1-C10、SY5Y、A431和HeLa细胞中连接素-1表达下调，HSV通过内吞作用进入细胞。相反，在HSV从质膜进入的Vero细胞上，nectin-1没有下调。对b78h1来源细胞的进一步分析表明，nectin-1的下调与gD结合nectin-1的能力相对应，并且是通过nectin-1的内化和低ph依赖性降解来实现的。此外，gD对于表达nectin-1的B78H1细胞的病毒粒子内化是必需的。这些数据表明，gd介导的nectin-1内化的决定因素可能在进入过程中引导HSV进入内吞途径。
During herpes simplex virus (HSV) entry, membrane fusion occurs either on the cell surface or after virus endocytosis. In both cases, binding of glycoprotein D (gD) to a receptor such as nectin-1 or HVEM is required. In this study, we co-cultured cells expressing gD with nectin-1 expressing cells to investigate the effects of gD on nectin-1 at cell contacts. After overnight co-cultures with gD expressing cells, there was a down-regulation of nectin-1 in B78H1-C10, SY5Y, A431 and HeLa cells, which HSV enters by endocytosis. In contrast, on Vero cells, which HSV enters at the plasma membrane, nectin-1 was not down-regulated. Further analysis of B78H1-derived cells showed that nectin-1 down-regulation corresponds to the ability of gD to bind nectin-1 and is achieved by internalization and low-pH-dependent degradation of nectin-1. Moreover, gD is necessary for virion internalization in B78H1 cells expressing nectin-1. These data suggest that the determinants of gD-mediated internalization of nectin-1 may direct HSV to an endocytic pathway during entry.