Delay of neutrophil apoptosis in acute coronary syndromes
Delay of neutrophil apoptosis in acute coronary syndromes
复制标题
DOI：
10.1189/jlb.0703358
复制
发表时间：
2004-05-01
影响因子：
5.5
通讯作者：
Daniel, WG
中科院分区：
文献类型：
--
作者：
Garlichs, CD;Eskafi, S;Daniel, WG
多形核中性粒细胞（PMN）的凋亡是目前讨论的一个关键事件，在控制炎症。本研究确定了对照组（C）、稳定型（SAP）或不稳定型心绞痛（UAP）患者和急性心肌梗死（AMI）患者的PMN凋亡及其潜在机制。从C、SAP、UAP和AMI各15名受试者中抽取血液。通过流式细胞术测量分离的PMN（碘化丙啶染色）和来自全血的PMN（CD 16，Fc γ RIII）中的细胞凋亡。酶联免疫吸附法测定血清细胞因子。急性冠状动脉综合征（ACS）患者PMN凋亡明显延迟（ACS组51.2 ± 12.6%; SAP组44.9 ± 13.6%; UAP组28.4 ± 10.0%; AMI组20.3 ± 8.5%; AMI或UAP组与SAP或C组相比，P
Apoptosis of polymorphonuclear neutrophils (PMN) is currently discussed as a key event in the control of inflammation. This study determined PMN apoptosis and its underlying mechanisms in controls (C), patients with stable (SAP) or unstable angina (UAP), and with acute myocardial infarction (AMI). Blood was drawn from 15 subjects of each C, SAP, UAP, and AMI. Apoptosis was measured by flow cytometry in isolated PMN (propidium iodide staining) and PMN from whole blood (CD 16, FcgammaRIII). Serum cytokines were determined by enzyme-linked immunosorbent assay. Apoptosis of isolated PMN was delayed significantly in acute coronary syndromes (ACS) as compared with SAP or C (C, 51.2 +/- 12.6%; SAP, 44.9 +/- 13.6%; UAP, 28.4 +/- 10.0%; AMI, 20.3 +/- 8.5 %; AMI or UAP vs. SAP or C, P