Ultra-high-performance liquid chromatography coupled to quadrupole orbitrap high-resolution mass spectrometry for multi-residue screening of pesticides, (veterinary) drugs and mycotoxins in edible insects
Ultra-high-performance liquid chromatography coupled to quadrupole orbitrap high-resolution mass spectrometry for multi-residue screening of pesticides, (veterinary) drugs and mycotoxins in edible insects
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DOI：
10.1016/j.foodchem.2019.04.082
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发表时间：
2019-09-30
期刊：
影响因子：
8.8
通讯作者：
Vanhaecke, Lynn
中科院分区：
文献类型：
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作者：
De Paepe, Ellen;Wauters, Jella;Vanhaecke, Lynn
建立了一种通用提取和UHPLC-Q-Orbitrap (TM)-HAMS方法，对四种昆虫（粉虫、蚱蜢、家蟋蟀和黑虻）进行了大范围的有机化学污染物分析，包括杀虫剂（n = 25）、（兽药）药物（n = 29）和真菌毒素（n = 23）。为了证明该方法“适合用途”，通过确定筛选检测限（SDL）、选择性和特异性进行了定性验证，并通过评估日内精度（相对标准偏差（RSD））和回收率进行了半定量验证。在粉虫、蚱蜢、家蟋蟀和黑虻中，分别检测到64、61、59和62种化合物的SDL水平（1 ~ 100 μ g kg(-1)），显著低于其他可食用基质的现有最大残留限量。平均加样回收率在70% ~ 120%之间，rsd值符合欧洲标准（CD 2002/657/EC； SANCO）。最后，筛选方法的潜力被证明在真实的昆虫样本，显示轻微或没有污染。
A generic extraction and UHPLC-Q-Orbitrap (TM)-HAMS method was developed for four insect species (mealworm, grasshopper, house cricket and black soldier fly) analyzing a large spectrum of organic chemical contaminants, including pesticides (n = 25), (veterinary) drugs (n = 29), and mycotoxins (n = 23). To prove the method as 'fitfor-purpose', a successful validation was performed, both qualitatively, by determining the screening detection limit (SDL), selectivity and specificity, as well as semi-quantitatively, by assessing the within-day precision (relative standard deviation (RSD)) and recovery. For both the mealworm, grasshopper, house cricket and black soldier fly, 64, 61, 59 and 62 compounds were detected at the respective SDL levels (1-100 mu g kg(-1)), predominantly below existing maximum residue limits for other edible matrices. Mean recoveries ranged between 70% and 120% and RSD-values were in line with European regulations (CD 2002/657/EC; SANCO). Finally, the potential of the screening methodology was demonstrated on real insect samples, revealing minor to no contamination.