Cell response to dextran-derivatised iron oxide nanoparticles post internalisation
Cell response to dextran-derivatised iron oxide nanoparticles post internalisation
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DOI：
10.1016/j.biomaterials.2003.12.046
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发表时间：
2004-10-01
期刊：
影响因子：
14
通讯作者：
Curtis, ASG
中科院分区：
文献类型：
--
作者：
Berry, CC;Wells, S;Curtis, ASG
磁性纳米颗粒多年来一直被用作磁共振成像造影剂。尽管目前有几种葡聚糖包被的氧化铁纳米颗粒在临床前和临床上使用，但关于这种颗粒对培养细胞的影响的信息很少。粒子用作造影剂的先决条件是捕获并随后被细胞摄取。这项研究涉及使用合成的磁性纳米颗粒和葡聚糖衍生的磁性纳米颗粒，并与类似的未塑化的普通颗粒进行比较。使用人皮肤成纤维细胞和各种观察细胞-颗粒相互作用的技术，包括光学和荧光显微镜、扫描和透射电子显微镜，在体外评估了这种影响。结果表明，尽管未包覆的和葡聚糖衍生的颗粒都被吸收到细胞内，但衍生的颗粒引起了与普通颗粒不同的细胞行为和形态的改变，这表明细胞的反应依赖于颗粒的涂层。(C)2003爱思唯尔有限公司。保留所有权利。
Magnetic nanoparticles have been used for many years as magnetic resonance imaging contrast agents. Despite the fact that there are currently several dextran-coated iron oxide nanoparticles are in preclinical and clinical use, there is very little information available concerning the influence such particles have on cells in culture. The prerequisite for particles employed as contrast agents is capture and subsequent uptake by cells. This study involved the use of magnetic nanoparticles synthesised and derivatised with dextran, compared to similar underivatised plain particles. The influence in vitro was assessed using human dermal fibroblasts and various techniques to observe cell-particles interaction, including light and fluorescence microscopy, scanning and transmission electron microscopy. The results indicate that although both the uncoated and the dextran-derivatised particles are uptaken into the cell, the derivatised particles induce alterations in cell behaviour and morphology distinct from the plain particles, suggesting that cell response is dependent on the particles coating. (C) 2003 Elsevier Ltd. All rights reserved.