Infection and Transovarial Transmission of Rickettsiae in Dermacentor variabilis Ticks Acquired by Artificial Feeding
Infection and Transovarial Transmission of Rickettsiae in Dermacentor variabilis Ticks Acquired by Artificial Feeding
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DOI：
10.1089/153036601750137660
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发表时间：
2001-03-01
影响因子：
2.1
通讯作者：
Azad, Abdu
中科院分区：
文献类型：
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作者：
Macaluso, Kevin;Sonenshine, Daniel;Azad, Abdu
在这项研究中，我们检验了利用玻璃微血管体外饲养技术作为建立立克次体感染蜱的方法的效率。为了量化蜱在微毛细血管摄食过程中的摄取量，我们计算了蜱肠道和血淋巴中放射性标记氨基酸的掺入量。18只蜱中有15只的摄取量在0.06 ~ 6.77 μ l之间。但毛细饲养时，液体摄取量与体重增加无关。未感染和部分喂养的实验室饲养的雌性变异性皮肤革螨蜱通过微毛细管暴露于蒙大拿立克次体或鼻甲立克次体感染的Vero细胞中，返回兔宿主，并允许其进食至饱足。当IFA检查时，所有从被允许在感染瑞克-埃茨病的液体上过夜的蜱身上收集的组织都被发现被感染。当感染立克次体和未感染立克次体的毛细血管喂养蜱虫被允许进食到饱腹并产卵时，在平均充血重量或繁殖力方面没有观察到显着差异。当我们用聚合酶链反应（PCR）和IFA方法评估吸入立克次体感染细胞的蜱的经生殖道传播效率时，暴露于蒙大拿恙螨的蜱的卵中有85%被PCR检测到感染，暴露于鼻管蜱的蜱的卵中有69%被PCR检测到感染。立克次体基因未在未感染的对照样本中扩增。IFA对接触立克次体感染细胞的雌性卵样本进行检查，100%的检测样本中发现立克次体，而未感染的对照为阴性。
In this study we examined the efficiency of an in vitro feeding technique using glass microcapillaries as a method of establishing rickettsiae-infected lines of ticks. To quantify the volume ingested by ticks during microcapillary feeding, the incorporation of radiolabeled amino acids in tick gut and hemolymph was calculated. Fifteen of 18 ticks consumed between 0.06 and 6.77 mu l. However, ingestion of fluid was not correlated to weight gain during capillary feeding. Uninfected and partially fed laboratory-reared female Dermacentor variabilis ticks were exposed to either Rickettsia montana-or Rickettsia rhipicephali-infected Vero cells via microcapillary tubes, returned to rabbit hosts, and allowed to feed to repletion. All tissues collected from ticks allowed to feed overnight on rick-ettsiae-infected fluids were found to be infected when examined by IFA. When rickettsiae-infected and uninfected capillary-fed ticks were allowed to feed to repletion and lay eggs, no significant differences in mean engorgement weight or fecundity was observed. When we assessed the efficiency of transovarial transmission of rickettsiae by ticks that imbibed rickettsiae-infected cells by polymerase chain reaction (PCR) and IFA, infection was detected by PCR in the eggs from 85% of the ticks exposed to R. montana and 69% of the ticks exposed to R. rhipicephali. Rickettsial genes were not amplified in samples of the uninfected controls. Examination by IFA of egg samples from females exposed to rickettsiae-infected cells identified rickettsiae in 100% of the samples tested, while the uninfected controls were negative.