Progesterone induction of chondroitin sulfate proteoglycan aggrecan expression in human endometrial epithelial cells.
Progesterone induction of chondroitin sulfate proteoglycan aggrecan expression in human endometrial epithelial cells.
复制标题
黄体酮诱导人子宫内膜上皮细胞中硫酸软骨素蛋白聚糖聚集蛋白聚糖的表达。
DOI：
10.1016/j.jsbmb.2010.07.004
复制
发表时间：
2010
影响因子：
4.1
通讯作者：
Kotaro Kitaya.
中科院分区：
文献类型：
--
作者：
Tadahiro Yasuo;Takeshi Yamaguchi;Kotaro Kitaya.
Chondroitin sulfate (CS) is the most abundant glycosaminoglycan species in the human endometrium, but the expression profile of CS proteoglycans (PGs) in this mucosal tissue remains fully undetermined. In this study, we aimed to clarify the expression of CSPGs including aggrecan, neurocan, melanoma-associated CSPG, neuroglycan C, and brevican in the human cycling endometrium. By reverse transcription-polymerase chain reaction, the gene transcripts for aggrecan core protein were detected in all samples examined, while other CSPGs were not. Western blotting showed the immunoreactivity for aggrecan core protein at approximately 370kDa size after enzymatic digestion of CS-A and CS-C side chains. The expression level of aggrecan core protein was significantly higher in the secretory phase than in the proliferative phase. The immunostaining for aggrecan was detected in the endometrial microvascular endothelium throughout the menstrual cycle. The immunostaining in the glandular epithelium was faint during the proliferative and early secretory phase, but distinct during the mid-to-late-secretory phase. Progesterone, but not 17β-estradiol, induced aggrecan core protein expression in cultured endometrial epithelial cells. The endometrial expression pattern of aggrecan was distinct from that of other known CSPGs, suggesting the unique role of this proteoglycan at the implantation site.
