QTL analysis for flowering time using backcross population between Oryza sativa Nipponbare and O. rufipogon
QTL analysis for flowering time using backcross population between Oryza sativa Nipponbare and O. rufipogon
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DOI：
10.1266/ggs.85.273
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发表时间：
2010-08-01
影响因子：
1.1
通讯作者：
Ishii, Takashige
中科院分区：
文献类型：
--
作者：
Pham Thien Thanh;Phuong Dang Thai Phan;Ishii, Takashige
在不久的将来，由于温室气体的积累，预计全球平均气温将上升，气温上升将导致许多作物品种严重不育。在水稻中，由于野生种表现出高度的遗传变异，它们可能具有改善栽培品种开花性状的潜力。在本研究中，我们研究了自然条件下的开花特性，通过比较亚洲野生稻资源Oryza rufipogon W630（来自缅甸）和日本栽培稻O.粳稻日本晴此外，利用来自它们之间杂交的BC2F8回交群体进行抽穗天数（DH）和小穗打开时间（SOT：一天中小穗打开的时间）的QTL分析。关于DH，检测到4个QTL，其中两个被发现有野生型等位基因，导致在日本夏季抽穗天数较长。这些野生等位基因可用于生产在未来预计的夏季高温期间不开花的迟抽穗品种。对于SOT，记录了第一个小穗开放的开始时间（SOTb）和50%小穗开放的中位时间（SOTm）两个参数，并研究了与日本晴的时间差异。SOTb和SOTm分别位于第5和第10染色体以及第4和第5染色体上。在所有位点上，野生等位基因都与小穗开放时间早有关。如果本研究中检测到的野生等位基因在其他品种的遗传背景中具有相同的效应，它们将非常有用，用于培育在温度上升之前的早晨完成受精的早花水稻品种。
In the near future, global average temperature is expected to increase due to the accumulation of greenhouse gases, and increased temperatures will cause severe sterility in many crop species. In rice, since wild species show high genetic variation, they may have the potential to improve the flowering characters of cultivars. In this study, we investigated flowering characters under natural conditions by comparing an Asian wild rice accession of Oryza rufipogon W630 (originated from Myanmar) with a Japanese rice cultivar, O. sativa Japonica cv. Nipponbare. Further, QTL analysis for days to heading (DH) and spikelet opening time (SOT: the time of day when the spikelet opens) was carried out using BC2F8 backcross population derived from the cross between them. Regarding DH, four QTLs were detected, and two of them were found to have wild alleles with strong effects leading to longer days to heading during the Japanese summer. These wild alleles may be used to produce late-heading cultivars that do not flower during the high summer temperatures anticipated in the future. As for SOT, two parameters of SOTb (beginning time when the first spikelet opens) and SOTm (median time when 50% of the spikelets open) were recorded and the time differences from Nipponbare were investigated. Two QTLs on chromosomes 5 and 10 and two QTLs on chromosomes 4 and 5 were detected for SOTb and SOTm, respectively. The wild alleles were responsible for early spikelet opening time at all loci. If the wild alleles detected in this study have the same effects in the genetic background of other cultivars, they will be very useful in producing early-flowering rice cultivars that complete fertilization in the morning before the temperature rises.