Gold nanorods-based thermosensitive hydrogel produces selective long-lasting regional anesthesia triggered by photothermal activation of Transient Receptor Potential Vanilloid Type-1 channels.
Gold nanorods-based thermosensitive hydrogel produces selective long-lasting regional anesthesia triggered by photothermal activation of Transient Receptor Potential Vanilloid Type-1 channels.
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DOI：
10.1016/j.colsurfb.2018.07.002
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Cheng Zhou;Jianqiong Huang;Qian Yang;Tao Li;Jin Liu;Z. Qian
中科院分区：
文献类型：
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作者：
Cheng Zhou;Jianqiong Huang;Qian Yang;Tao Li;Jin Liu;Z. Qian
长时间的区域麻醉和选择性感觉阻滞在术后镇痛和病理性疼痛的治疗中是有用的。先前的研究表明，TRPV 1（瞬时受体电位香草酸1型）通道的激活促进了QX-314在体内选择性持久区域麻醉的效力。水凝胶是一种固体的类水凝胶材料，具有从软而弱到硬而坚韧的广泛性质。金纳米棒是纳米颗粒，可通过暴露于近红外辐射用于热疗。我们制备了含金纳米棒和QX-314的水凝胶。调节水凝胶的分子量以实现目标相变温度。将具有所需光热转换效率的金纳米棒和QX-314与水凝胶混合以产生金纳米棒-QX-314/水凝胶纳米复合物。采用大鼠坐骨神经阻滞模型评价金纳米棒-QX-314/水凝胶纳米复合材料的局部麻醉作用。在近红外辐射下，金纳米棒-QX-314/水凝胶纳米复合物通过光热转换激活TRPV 1通道，同时释放QX-314。金纳米棒和QX-314负载水凝胶表现出持久的区域麻醉作用，具有选择性感觉功能阻滞。纳米复合材料的感觉阻滞时间明显长于1%利多卡因（90.0 ± 12.2vs. 37.5± 12.5min，P < 0.01）。只有40%的大鼠观察到纳米复合材料的运动阻滞，其持续时间明显短于感觉阻滞（42.5 ± 17.1vs. 90.0± 12.2min，P < 0.01）。金纳米棒-QX-314/水凝胶纳米复合材料在大鼠坐骨神经阻滞模型中可产生选择性长激光区域麻醉作用。
Long-lasting regional anesthesia and selective sensory block are useful in post-operative analgesia and treatment of pathological pain. Previous studies have demonstrated that activation of TRPV1 (Transient Receptor Potential Vanilloid Type-1) channels facilitated the potency of QX-314 for selective long-lasting regional anesthesiain vivo. Hydrogel is a solid jelly-like material covering a wide range of properties from soft and weak to hard and tough. Gold nanorods are nanoparticles, which can be used for hyperthermia by exposure to near-infrared radiation. We fabricated a gold nanorods and QX-314 containing hydrogel. The molecular weight of hydrogel was adjusted to achieve a targeted phase transition temperature. Gold nanorods with a desired photothermal conversion efficacy and QX-314 were mixed with hydrogel to produce a gold nanorods-QX-314/hydrogel nanocomposite. A rat model of sciatic nerve block was applied to evaluate the regional anesthetic effect of the gold nanorods-QX-314/hydrogel nanocomposite. Upon exposure to near-infrared irradiation, the gold nanorods-QX-314/hydrogel nanocomposite activated TRPV1 channels through photothermal conversion and release of QX-314 at the same time. The gold nanorods and QX-314 loaded hydrogel exhibited a long-lasting regional anesthetic effect with selective sensory function block. Sensory block duration of the nanocomposite was significantly longer than of 1% lidocaine (90.0 ± 12.2vs. 37.5 ± 12.5 min, P < 0.01). Motor block by the nanocomposite was observed for only 40% of rats with significantly shorter duration than its sensory block (42.5 ± 17.1vs. 90.0 ± 12.2 min, P < 0.01). The gold nanorods-QX-314/hydrogel nanocomposite can produce a selective long-lasing regional anesthetic effect in a rat model of sciatic nerve block.