Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A.
Isolation and properties of cell lines from the metastasising rat mammary tumour SMT-2A.
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DOI：
10.1038/bjc.1989.182
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发表时间：
1989-06
影响因子：
8.8
通讯作者：
Monaghan, P
中科院分区：
文献类型：
--
作者：
Rudland, P S;Dunnington, D J;Kim, U;Gusterson, B A;O'Hare, M J;Monaghan, P
通过逐步使肿瘤细胞适应组织培养，从腹水型SMT-2A转移性大鼠乳腺肿瘤中分离出一种新的细胞系Rat mammary（拉马）900。细胞主要以松散粘附的聚集体形式生长，并且在体外的前18代期间依赖于从相同肿瘤获得的间皮瘤样细胞（拉马950）的饲养层。皮下注射拉马900细胞在同系Wistar Furth大鼠的脂肪垫产生间变性原发肿瘤和广泛的，肉眼可见的转移，包括在肺，淋巴结，肝脏和骨骼，类似于父母的可移植肿瘤。对于拉马900细胞，通过体外传代17次，皮下脂肪垫的转移扩散程度增加。通过在体内注射原始细胞和非致瘤性拉马950细胞，在早期达到了相似程度的转移扩散。皮下注射拉马900到胸腺切除大鼠或MF 1 nu/nu小鼠产生较少的肿瘤，其中大部分消退。在胸腺切除大鼠中没有发生转移，在裸鼠中观察到较少的转移，主要在肺中，但不在淋巴结、肝或骨中。通过腹腔注射裸鼠形成的腹水瘤含有类似于拉马900的大鼠间变性细胞和类似于拉马950的小鼠间皮样细胞。尽管这些间变性腹水细胞在同基因或胸腺切除的大鼠中未能产生任何肿瘤，但它们仍然在裸鼠中产生肿瘤和转移，包括淋巴结中的肿瘤和转移。
A new cell line Rat mammary (Rama) 900 was isolated from the ascitic version of the SMT-2A metastasising rat mammary tumour by stepwise adaptation of the tumour cells to tissue culture. The cells grew mainly as loosely-adherent aggregates, and were dependent during the first 18 passages in vitro on a feeder layer of mesothelial-like cells (Rama 950) obtained from the same tumour. Subcutaneous injection of Rama 900 cells in fat pads of syngeneic Wistar Furth rats yielded anaplastic primary tumours and extensive, gross metastases including those in lungs, lymph nodes, liver and bones, similar to the parental transplantable tumour. The extent of metastatic spread from subcutaneous fat pads was increased by passage 17 in vitro for the Rama 900 cells. A similar extent of metastatic spread was achieved at earlier times by injecting the original cells with the non-tumorigenic Rama 950 cells in vivo. Subcutaneous injection of Rama 900 into thymectomised rats or MF1 nu/nu mice yielded fewer tumours, most of which regressed. No metastases occurred in the thymectomised rats and fewer metastases, mainly in lungs but not in lymph nodes, livers or bones, were seen in the nude mice. The ascitic tumours formed by intraperitoneal injection of nude mice contained both anaplastic rat cells similar to Rama 900 and mouse mesothelial-like cells similar to Rama 950. Although these anaplastic ascites cells failed to yield any tumours in syngeneic or thymectomised rats, they still produced tumours and metastases, including those in lymph nodes, in nude mice.