Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines
Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines
复制标题
DOI：
10.1016/j.actbio.2009.09.024
复制
发表时间：
2010-05-01
影响因子：
9.7
通讯作者：
Hort, Norbert
中科院分区：
文献类型：
--
作者：
Feyerabend, Frank;Fischer, Janine;Hort, Norbert
生物医用可降解镁合金即将进入临床应用。稀土元素（RE）用于改善合金的机械性能，但或多或少是不确定的混合物。对于这一组中的某些元素，关于毒性和对细胞影响的数据很少。因此，在本研究中，通过与氯化物一起孵育，评价了元素钇（Y）、钕（Nd）、镝（Dy）、镨（Pr）、钆（Gd）、镧（La）、铈（Ce）、铕（Eu）、锂（Li）和锆（Zr）的体外细胞毒性（10-2000 μ M）;镁（Mg）和钙（Ca）在较高浓度（分别为200和50 mM）下进行测试。测定了对人骨肉瘤细胞系MG 63、人脐带血管周围（HUCPV）细胞和小鼠巨噬细胞（RAW 264.7）活力的影响，以及对细胞凋亡和炎症因子（TNF-α、IL-1 α）表达的诱导。可以观察到应用的电池之间的显著差异。RAW的敏感性最高，HUCPV的敏感性最低。La和Ce显示出最高的细胞毒性的分析元素。在镁合金中溶解度高的元素中，Gd和Dy似乎比Y更合适。镁合金生物医学应用开发的重点应包括具有众所周知的组织特异性和全身效应的大多数定义的合金成分。(C)2009 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Degradable magnesium alloys for biomedical application are on the verge of being used clinically. Rare earth elements (REEs) are used to improve the mechanical properties of the alloys, but in more or less undefined mixtures. For some elements of this group, data on toxicity and influence on cells are sparse. Therefore in this study the in vitro cytotoxicity of the elements yttrium (Y), neodymium (Nd), dysprosium (Dy), praseodymium (Pr), gadolinium (Gd), lanthanum (La), cerium (Ce), europium (Eu), lithium (Li) and zirconium (Zr) was evaluated by incubation with the chlorides (10-2000 mu M); magnesium (Mg) and calcium (Ca) were tested at higher concentrations (200 and 50 mM, respectively). The influence on viability of human osteosarcoma cell line MG63, human umbilical cord perivascular (HUCPV) cells and mouse macrophages (RAW 264.7) was determined, as well as the induction of apoptosis and the expression of inflammatory factors (TNF-alpha, IL-1 alpha). Significant differences between the applied cells could be observed. RAW exhibited the highest and HUCPV the lowest sensitivity. La and Ce showed the highest cytotoxicity of the analysed elements. Of the elements with high solubility in magnesium alloys, Gd and Dy seem to be more suitable than Y. The focus of magnesium alloy development for biomedical applications should include most defined alloy compositions with well-known tissue-specific and systemic effects. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.