Size and number of nuclei differ in normal and neoplastic acinar cells from rat pancreas.
Size and number of nuclei differ in normal and neoplastic acinar cells from rat pancreas.
复制标题
大鼠胰腺的正常腺泡细胞和肿瘤性腺泡细胞的细胞核大小和数量不同。
DOI：
10.1097/00006676-198601000-00008
复制
发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Longnecker,DS
中科院分区：
文献类型：
--
作者：
Morgan,RG;Schaeffer,BK;Longnecker,DS
腺泡细胞通过酶消化从正常胰腺和从阿扎丝氨酸诱导的非典型腺泡细胞结节和可移植的腺泡细胞癌中分离。正常腺泡细胞以双核为主，细胞质丰富。它们的直径为10-24 μ m，尺寸分布偏向较小的尺寸，但峰值在18 μ m。从41个去核结节中分离细胞，重量从1.1到200 mg不等。这些细胞主要是单核细胞，尺寸比正常细胞更均匀，直径在9 μ m处达到峰值。研究的所有结节的细胞大体相似，结节大小和单核化程度之间没有关系。来自可移植肿瘤的细胞很小，几乎没有细胞质，并且几乎完全是单核细胞。还在处死前4个月注射阿扎胞苷的大鼠胰腺切片中研究了正常和显微镜下非典型腺泡细胞结节的双核化程度。这些切片中的细胞核和细胞计数证实，正常组织中的双核比结节组织中的双核更常见。这些研究强调了在正常胰腺腺泡细胞中发现的高度双核化。他们证明了使用细胞分离技术从正常和肿瘤胰腺组织中获得腺泡细胞制剂的可行性，用于研究这些细胞的功能和形态差异。
Acinar cells were isolated by enzymatic digestion from normal pancreas and from azaserine-induced atypical acinar cell nodules and a transplant-able acinar cell carcinoma. Normal acinar cells were predominately binuclear, with abundant cytoplasm. They were 10-24 pm in diameter, with a size distribution skewed toward smaller sizes but with a peak at 18 [mu] m. Cells were isolated from 41 enucleated nodules varying in weight from 1.1 to 200 mg. These cells were predominantly mononuclear, with a more uniform size than normal cells and a peak at 9 pm diameter. Cells from all nodules studied were grossly similar, and there was no relation between nodule size and the degree of mononucleation. Cells from the transplantable tumor were small, with little cytoplasm, and were almost exclusively mononuclear. The extent of binucleation in normal and microscopic atypical acinar cell nodules was also studied in sections from the pancreas of rats injected with azaserine 4 months before killing. Nuclear and cell counts in these sections confirmed that binucleation is more frequent in normal than in nodule tissue. These studies emphasize the high degree of binucleation found in normal pancreatic acinar cells. They demonstrate the feasibility of using cell separation techniques to obtain preparations of acinar cells from normal and neoplastic pancreatic tissue for studies of functional and morphological differences in these cells.