Orbital Shear Stress Regulates Differentiation and Barrier Function of Primary Renal Tubular Epithelial Cells.
Orbital Shear Stress Regulates Differentiation and Barrier Function of Primary Renal Tubular Epithelial Cells.
复制标题
轨道剪切应力调节原发性肾小管上皮细胞的分化和屏障功能。
DOI：
10.1097/mat.0000000000000723
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Fissell WH
中科院分区：
文献类型：
--
作者：
Ferrell N;Cheng J;Miao S;Roy S;Fissell WH
体外培养的原代细胞逐渐失去体内表型的特征。有助于维持细胞特异性表型的培养技术有利于组织工程和生物人工器官的发育。在这里，我们通过在轨道振动器上培养细胞来评估受流体剪切应力影响的人肾小管上皮细胞的表型。在眶切应力作用下的细胞中测量了经上皮电阻、细胞密度以及近端小管特异性功能标志物的基因和蛋白表达。在轨道振动器上培养的细胞经上皮电阻增加，细胞密度增加，小管上皮特异性基因和蛋白表达增强。这可能至少部分是由于施加在细胞顶端表面的机械应力，尽管其他因素，包括增加的营养和氧气输送以及改善的混合也可能起作用。这些结果表明，轨道振动器培养可能是一种简单的方法来增加培养的肾上皮细胞的分化表型。
Primary cells cultured in vitro gradually lose features characteristic of the in vivo phenotype. Culture techniques that help maintain cell-specific phenotype are advantageous for development of tissue engineered and bioartificial organs. Here we evaluated the phenotype of primary human renal tubule epithelial cells subjected to fluid shear stress by culturing the cells on an orbital shaker. Transepithelial electrical resistance, cell density, and gene and protein expression of proximal tubule specific functional markers were measured in cells subjected to orbital shear stress. Cells cultured on an orbital shaker had increased transepithelial electrical resistance, higher cell density, and enhanced tubular epithelial specific gene and protein expression. This is likely due at least in part to the mechanical stress applied to the apical surface of the cells, although other factors including increased nutrient and oxygen delivery and improved mixing could also play a role. These results suggest that orbital shaker culture may be a simple approach to augmenting the differentiated phenotype of cultured renal epithelial cells.