Polydopamine coatings enhance biointegration of a model polymeric implant
Polydopamine coatings enhance biointegration of a model polymeric implant
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DOI：
10.1039/c1sm05918a
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发表时间：
2011-01-01
期刊：
影响因子：
3.4
通讯作者：
Kohane, Daniel S.
中科院分区：
文献类型：
--
作者：
Jeong, Kyung Jae;Wang, Liqiang;Kohane, Daniel S.
植入物的生物整合影响其功能、稳定性和安全性。尽管该主题的大多数研究都集中在骨骼和其他硬组织上，但与软组织的生物整合在许多应用中都很重要，例如在假体角膜中。在这里，我们采用了基于聚多巴胺的粘合表面化学，以增强通常用于假体角膜的模型聚合物聚甲基丙烯酸甲酯（PMMA）与软组织的生物整合。聚多巴胺涂层（PDA）和随后用细胞粘附肽RGD（PDA-PEG-RGD）进行的修饰显着增强了角膜上皮细胞和角膜细胞的增殖，而不会导致任一细胞类型过度分泌促炎细胞因子（例如IL-6）。 PDA 与胶原凝胶紧密粘附，而 PDA-PEG-RGD 和未涂覆的 PMMA 则不然。通过在血清中预孵育，PDA 对胶原蛋白的粘附力大大降低。皮下植入 45 天后，两个聚多巴胺涂层表面的组织反应均为良性。然而，与胶原凝胶的研究结果相反，PDA-PEG-RGD 与组织的结合比 PDA 更紧密，尽管两者的结合都比未改性的 PMMA 更好。基于聚多巴胺的表面化学物质可能有助于增强植入物与软组织的组织整合。
The biointegration of implants affects their function, stability and safety. Although most research on this topic has focused on bone and other hard tissues, biointegration with soft tissues is important in numerous applications, such as in prosthetic corneas. Here, we have adapted polydopamine-based adhesive surface chemistry to enhance the biointegration with soft tissue of a model polymer-poly (methyl methacrylate) (PMMA), commonly used in prosthetic corneas. Polydopamine coating (PDA) and subsequent modification with the cell-adhesive peptide RGD (PDA-PEG-RGD) significantly enhanced cellular proliferation of corneal epithelial cells and keratocytes without causing excessive secretion of pro-inflammatory cytokines (e. g. IL-6) by either cell type. PDA adhered tightly to collagen gels, while PDA-PEG-RGD and uncoated PMMA did not. PDA's adhesion to collagen was greatly reduced by preincubation in serum. Tissue reaction to both polydopamine-coated surfaces was benign after 45 days of subcutaneous implantation. However, in contrast to the findings with collagen gels, PDA-PEG-RGD bound much more tightly to tissue than did PDA-although both bound better than unmodified PMMA. Polydopamine-based surface chemistries are potentially useful in enhancing tissue integration of implants with soft tissues.