Imaging flow cytometry for the study of erythroid cell biology and pathology.
Imaging flow cytometry for the study of erythroid cell biology and pathology.
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DOI：
10.1016/j.jim.2015.03.019
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发表时间：
2015-08
影响因子：
2.2
通讯作者：
McCoy JP Jr
中科院分区：
文献类型：
--
作者：
Samsel L;McCoy JP Jr
红系细胞成熟和影响红细胞的疾病通常伴随着这些细胞的形态学和免疫表型变化。在过去，这些变化主要是通过使用手动显微镜进行评估，这大大限制了这些研究的统计严谨性，吞吐量和客观性。成像流式细胞术提供了一种技术来检查细胞的形态以及以统计学上稳健的方式在逐个细胞的基础上以高通量量化许多荧光标记物的染色强度和信号分布，因此非常适合于研究红细胞生物学。迄今为止，成像流式细胞术已用于研究三个领域的红细胞：1）红细胞成熟，2）镰状细胞病，和3）疟疾等传染病。在成熟研究中，成像流式细胞术可以紧密地概括已知的成熟阶段，并导致鉴定新的红系细胞前体群体。在镰状细胞病中，成像流式细胞术提供了一种可靠的方法来量化镰状红细胞并识别与发病率相关的细胞聚集体，在疟疾中，成像流式细胞术已用于筛选新的化疗药物。这些研究表明了成像流式细胞术在红细胞生物学和病理学研究中的价值。
Erythroid cell maturation and diseases affecting erythrocytes are frequently accompanied by morphologic and immunophenotypic changes to these cells. In the past, these changes have been assessed primarily through the use of manual microscopy, which substantially limits the statistical rigor, throughput, and objectivity of these studies. Imaging flow cytometry provides a technology to examine both the morphology of cells as well as to quantify the staining intensity and signal distribution of numerous fluorescent markers on a cell-by-cell basis with high throughput in a statistically robust manner, and thus is ideally suited to studying erythroid cell biology. To date imaging flow cytometry has been used to study erythrocytes in three areas: 1) erythroid cell maturation, 2) sickle cell disease, and 3) infectious diseases such as malaria. In the maturation studies, imaging flow cytometry can closely recapitulate known stages of maturation and has led to the identification of a new population of erythroid cell precursors. In sickle cell disease, imaging flow cytometry provides a robust method to quantify sickled erythrocytes and to identify cellular aggregates linked to morbidities, and in malaria, imaging flow cytometry has been used to screen for new chemotherapeutic agents. These studies have demonstrated the value of imaging flow cytometry for investigations of erythrocyte biology and pathology.