Reduced expression of claudin-7 correlates with invasion and metastasis in squamous cell carcinoma of the esophagus
Reduced expression of claudin-7 correlates with invasion and metastasis in squamous cell carcinoma of the esophagus
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DOI：
10.1016/j.humpath.2005.12.018
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发表时间：
2006-05-01
期刊：
影响因子：
3.3
通讯作者：
Yokozaki, H
中科院分区：
文献类型：
--
作者：
Usami, Y;Chiba, H;Yokozaki, H
密蛋白是一种跨膜蛋白，可密封紧密连接，与外周蛋白闭合小带 (ZO)-1 结合，已知在多种正常组织和癌症中发挥重要作用。然而，claudin-1和claudin-7表达在食管鳞状细胞癌中的作用仍有待阐明。在本研究中，我们证实了claudin-1、claudin-7和ZO-1在正常人食管鳞状上皮的棘细胞层中的表达。采用免疫组织化学方法分析食管鳞状细胞癌浸润前部claudin-1和claudin-7的表达，以阐明其在肿瘤进展中的作用。食管癌浸润前部 Claudin-7 表达的减少与浸润深度 (P = .004)、分期 (P = .038)、淋巴管浸润 (P = .001) 和淋巴结转移 (P = .014) 显着相关。相反，除了肿瘤的组织学分化之外，claudin-1 表达与临床病理因素之间未检测到显着关联 (P = .0029)。比较原发肿瘤浸润前部及其相应转移淋巴结的claudin-7表达，发现转移淋巴结中claudin-7表达显着降低（P = .007）。这些结果表明食管鳞状细胞癌浸润前部claudin-7表达的减少可能导致肿瘤进展和随后的转移事件。因此，claudin-7可以成为预测淋巴结转移的新标志物。 (c) 2006 Elsevier Inc. 保留所有权利。
Claudins are transmembrane proteins that seal tight junctions, bind with peripheral protein zonula occludens (ZO)-1, and are known to play an important role in several normal tissues and cancers. However, the role of claudin-1 and claudin-7 expressions in esophageal squamous cell carcinoma remains to be clarified. In the present study, we confirmed the expressions of claudin-1, claudin-7, and ZO-1 in the prickle cell layer of the normal human esophageal squamous epithelium. The expressions of claudin-1 and claudin-7 at the invasive front of the esophageal squamous cell carcinoma were analyzed immunohistochemically to clarify their role in tumor progression. Reduced expression of claudin-7 at the invasive front of the esophageal cancer was significantly associated with the depth of invasion (P = .004), stage (P = .038), lymphatic vessel invasion (P = .001), and lymph node metastasis (P = .014). In contrast, significant association was not detected between claudin-1 expression and clinicopathologic factors except for histologic differentiation of the tumor (P = .0029). Comparison of claudin-7 expression at the invasive front of the primary tumor and its corresponding metastatic lymph nodes revealed significant reduction in claudin-7 expression in the metastatic lymph nodes (P = .007). These results Suggest that the reduced expression of claudin-7 at the invasive front of esophageal squamous cell carcinoma may lead to tumor progression and subsequent metastatic events. Thus, claudin-7 can be a novel marker for the prediction of lymph node metastasis. (c) 2006 Elsevier Inc. All rights reserved.