A PCR Method to Detect Nocardia seriolae in Fish Samples
A PCR Method to Detect Nocardia seriolae in Fish Samples
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鱼样中诺卡氏菌的PCR检测方法
DOI：
10.3147/jsfp.38.93
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发表时间：
2003
期刊：
影响因子：
0.6
通讯作者：
S. Suzuki
中科院分区：
文献类型：
--
作者：
Y. Miyoshi;S. Suzuki
建立了针对Nocardia seriolae 16 SrRNA基因的种特异性PCR（polymerase chain reaction，PCR）方法，以609 ~ 1038（Escherichia coli编号）核苷酸为靶点，扩增产物为432 bp。该方法可以检测N. seriolaetype strain（JCM 3360）和8株从黄尾Seriola quinqueradiata和日本比目鱼Paralichthys olivaceus中分离到的临床分离物，而未分离到其它5种诺卡氏菌。和4种黄尾病毒。该方法的最低检测限为102 CFU。用PCR方法对8只患病黄尾鱼进行了检测。所有鱼均获得阳性结果。
Species-specific polymerase chain reaction (PCR) targeting the 16S rRNA gene of Nocardia seriolae was developed.The PCR targeted nucleotide #609 to 1038 (Escherichia coli numbering), which gave a 432 bp-length product. This method could detectN. seriolaetype strain (JCM3360) and eight clinical isolates of this species from yellowtailSeriola quinqueradiataand Japanese flounder Paralichthys olivaceus, but not those of other bacterial species including 5 otherNocardiaspp. and 4 yellowtail pathogens. The detection limit of the PCR was 102CFU. Eight diseased yellowtail were employed for detection of the bacterium by the PCR. Positive results were obtained from all fish.