Proteomic analysis of injured spinal cord tissue proteins using 2-DE and MALDI-TOF MS
Proteomic analysis of injured spinal cord tissue proteins using 2-DE and MALDI-TOF MS
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DOI：
10.1002/pmic.200500621
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发表时间：
2006-05-01
期刊：
影响因子：
3.4
通讯作者：
Kim, Cheul Hong
中科院分区：
文献类型：
--
作者：
Kang, Soo Kyung;So, Hyeun Hwa;Kim, Cheul Hong
脊髓损伤（SCI）通过复杂和进化的分子级联反应诱导进行性病理生理，影响细胞存活和神经完整性，其相互关系尚不完全清楚。脊髓急性损伤发生一系列病理改变，包括出血、水肿、轴突和神经元坏死以及脱髓鞘。在本研究中，我们旨在利用2-DE和基质辅助激光解吸/电离- tof质谱（MALDI-TOF质谱）建立创伤性损伤脊髓组织中总蛋白表达的蛋白质组学图谱和特征。我们用2-DE和MS进行了蛋白质组学分析，描述了正常和创伤损伤脊髓组织的总蛋白和差异蛋白表达。该研究共发现了947种蛋白质，并分别从正常组织和受伤组织中分析了219种和270种蛋白质。创伤性损伤诱导24 h后，损伤脊髓组织中神经丝轻链、膜联蛋白5、热休克蛋白、微管蛋白β、外周蛋白、胶质纤维酸性蛋白δ、过氧化物还蛋白2、载脂蛋白a等39种蛋白表达上调，21种蛋白表达下调。大部分调节蛋白属于13个功能类别。在损伤组织中被鉴定为神经功能类别的蛋白质被认为最有可能参与伤口愈合反应以及神经发生和胶质瘤发生。
Spinal cord injury (SCI) induces a progressive pathophysiology affecting cell survival and neurological integrity via complex and evolving molecular cascades whose interrelationships are not fully understood. Acute injury to the spinal cord undergoes sequential pathological change including hemorrhage, edema, axonal and neuronal necrosis, and demyelination. In the present study, we aimed to establish the proteomic profiles and characterization of the total protein expressed in traumatic injured spinal cord tissue by using 2-DE and matrix assisted laser desorption/ionization-TOF MS (MALDI-TOF MS). We performed proteomic analysis using 2-DE and MS to describe total proteins and differential proteins expression between normal and traumatic injured spinal cord tissues. The study discovered 947 total proteins and analyzed 219 and 270 proteins from normal and injured tissue, respectively. After 24 h of traumatic damage induction, the injured spinal cord tissue up-regulated over 39 proteins including neurofilament light chain, annexin 5, heat shock protein, tubulin beta, peripherin, glial fibrillary acidic protein delta, peroxiredoxin 2, and apolipoprotein A. Twenty-one proteins showed reduction. The majority of the modulated proteins belonged to the 13 functional categories. Proteins that were identified with neural functional category in injured tissue were considered most likely to be involved in wound healing response coupled with neurogenesis and gliogenesis.