Galectin-related protein from Oreochromis niloticus: Molecular, and transcriptional features and biological responses against microbial pathogens
Galectin-related protein from Oreochromis niloticus: Molecular, and transcriptional features and biological responses against microbial pathogens
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DOI：
10.1016/j.aqrep.2021.100782
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发表时间：
2021-11
影响因子：
3.7
通讯作者：
Guoling Luo;Jinzhong Niu;J. Fang;Jufen Tang;Yishan Lu;Yu Huang;J. Jian
中科院分区：
文献类型：
--
作者：
Guoling Luo;Jinzhong Niu;J. Fang;Jufen Tang;Yishan Lu;Yu Huang;J. Jian
Galectin is a kind of lectin that can promote the cell adhesion, proliferation, and phagocytosis and participates in immune regulation and destruction of microbes. In this study, a galectin-related protein (On-GRP) homolog was identified from Nile tilapia, and its expression level and antibacterial ability were studied. The Open Reading Frame, which encoded a 179 amino acid protein (GenBank accession no: XM_005733705.1), was 537 bp. TheOn-GRPpossessed a carbohydrate recognition domain. qRT-PCR showed that theOn-GRPwas detected in the examined tissues in healthy Nile tilapia, and the highest expression levels was observed in the skin. After challenge withStreptococcus agalactiae, theOn-GRPexpression was up-regulated significantly (P < 0.05) in the whole blood, head kidney, gills and thymus. In addition, the recombinantOn-GRP(rOn-GRP) could agglutinate and bindStreptococcus agalactiaeandAeromonas hydrophila. In vitro and in vivo bacteriostatic tests showed that the rOn-GRP could inhibit the growth of bacteria. Besides, the rOn-GRP could promote the phagocytic ability of macrophages to engulf bacteria. Over all, this study indicatedOn-GRPis involved in the immune responses of Nile tilapia against bacterial pathogens.
