ACUTE AND CHRONIC EFFECT OF DIETARY CHOLIC-ACID ON COLONIC EPITHELIAL-CELL PROLIFERATION
ACUTE AND CHRONIC EFFECT OF DIETARY CHOLIC-ACID ON COLONIC EPITHELIAL-CELL PROLIFERATION
复制标题
DOI：
10.1159/000198579
复制
发表时间：
1981-01-01
期刊：
影响因子：
3.2
通讯作者：
RAICHT, RF
中科院分区：
文献类型：
--
作者：
DESCHNER, EE;COHEN, BI;RAICHT, RF
与对照组或0.2%胆酸喂养大鼠相比，雄性Fisher大鼠连续3天给予胆酸（1.0%饲料）导致每个结肠隐窝柱的DNA合成上皮细胞数量增加。隐窝的中间三分之一是被刺激以贡献额外增殖细胞的区域。3 H-TdR [氚化胸苷胸腺嘧啶脱氧核糖]标记细胞的最大数量增加了一倍，24小时和迁移进一步进行了1%的胆酸喂养大鼠的结肠隐窝比0.2%的胆酸或对照动物。与胆酸剥夺大鼠相比，长期饮食摄入0.2%胆酸（26周）增加了每柱标记细胞的数量，并扩大了增殖区室。在MNU[N-甲基-N-亚硝基脲]诱导的消耗胆酸的大鼠中结肠肿瘤的增强表现（先前报道）似乎与响应于胆汁酸对粘膜的有害作用而引起的细胞增殖水平升高有关。在胆酸处理的动物中进行DNA合成的上皮细胞数量增加将允许大肠中恶性转化的早期表达。
Administration of cholic acid (1.0% of the diet) to male Fisher rats for 3 days resulted in increased numbers of DNA synthesizing epithelial cells per colonic crypt column as compared to those found in control or 0.2% cholic acid-fed rats. The middle third of the crypt was the area stimulated to contribute the additional proliferating cells. The maximum number of 3H-TdR[tritiated thymidine thymine deoxyribose]-labeled cells was doubled by 24 h and migration had proceeded further up the colonic crypt of the 1% cholic acid-fed rats than the 0.2% cholic acid or control animals. Compared with cholic acid-deprived rats, long-term dietary intake of 0.2% cholic acid (26 wk) heighten the numbers of labeled cells per column and expand the proliferative compartment. The enhanced manifestation of colonic neoplasia in MNU[N-methyl-N-nitrosourea]-induced rats consuming cholic acid (previously reported) appears related to the elevated levels of cell proliferation brought about in response to the deleterious action of the bile acid on the mucosa. Increased numbers of epithelial cells undergoing DNA synthesis in cholic acid-treated animals would allow the earlier expression of malignant transformation in the large intestine.