Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis
Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis
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DOI：
10.1242/dev.060160
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发表时间：
2011-06-01
期刊：
影响因子：
4.6
通讯作者：
Kuehl, Michael
中科院分区：
文献类型：
--
作者：
Bugner, Verena;Tecza, Aleksandra;Kuehl, Michael
非洲爪蟾卵母细胞具有大量的母体核糖体储存，从而使早期发育与细胞生长所需的从头核糖体生物合成脱钩。含有Brix结构域的蛋白质，例如彼得潘（PPan），对于真核核糖体生物合成至关重要。在这项研究中，我们表明，PPan表达的母体以及在眼睛和颅神经嵴细胞（NCCs）在非洲爪蟾早期发展。耗尽PPan和干扰rRNA加工使用反义吗啉代寡核苷酸导致眼和颅软骨畸形。PPan的丢失，但不干扰rRNA加工，导致眼睛的特定标记基因（包括Rx1和Pax6）和NCC（如Twist、Slug和FoxD3）的早期下调。我们发现PPan蛋白定位于核仁和线粒体中，PPan的缺失导致细胞凋亡增加。这些发现表明PPan的一种新功能，它独立于其在核糖体生物合成中的作用。
The Xenopus oocyte possesses a large maternal store of ribosomes, thereby uncoupling early development from the de novo ribosome biosynthesis required for cell growth. Brix domain-containing proteins, such as Peter Pan (PPan), are essential for eukaryotic ribosome biogenesis. In this study, we demonstrate that PPan is expressed maternally as well as in the eye and cranial neural crest cells (NCCs) during early Xenopus laevis development. Depletion of PPan and interference with rRNA processing using antisense morpholino oligonucleotides resulted in eye and cranial cartilage malformations. Loss of PPan, but not interference with rRNA processing, led to an early downregulation of specific marker genes of the eye, including Rx1 and Pax6, and of NCCs, such as Twist, Slug and FoxD3. We found that PPan protein is localized in the nucleoli and mitochondria and that loss of PPan results in increased apoptosis. These findings indicate a novel function of PPan that is independent of its role in ribosome biogenesis.