Identification of novel odorant binding protein genes and functional characterization of OBP8 in Chilo suppressalis (Walker)
Identification of novel odorant binding protein genes and functional characterization of OBP8 in Chilo suppressalis (Walker)
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二化螟 (Walker) 中新型气味结合蛋白基因的鉴定和 OBP8 的功能表征
DOI：
10.1016/j.gene.2016.06.052
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发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
Dong Shuang-Lin
中科院分区：
文献类型：
--
作者：
Yang Ke;Liu Yan;Niu Dong-Juan;Wei Dan;Li Fei;Wang Gui-Rong;Dong Shuang-Lin
在昆虫嗅觉的外周水平，气味结合蛋白（OBP）被认为可以将外源性疏水性挥发物结合并转运到位于嗅觉神经元树突膜上的气味受体（OR）。本研究克隆了水稻二化螟29个OBP基因的cDNA序列，其中15个为新报道基因。通过RT-PCR检测了这些CsupOBPs的组织和时间表达模式，发现CsupOBPs 8、10和24在幼虫期的头部和成虫期的触角中特异表达。CsupOBP 6、9和18在幼虫期头部的表达量明显高于腹部，而CsupPBP 4和CsupOBP 1、3、8、11和24在成虫期两性触角中特异表达。这种表达谱强烈表明这些基因的嗅觉作用。此外，选择CsupOBP 8的功能通过竞争性荧光结合测定来研究。结果表明，CsupOBP 8与植物挥发性物质β-紫罗酮、橙花叔醇、法呢醇和2-己酮具有较高的结合亲和力（Ki = 4.9-15.0 μM），表明CsupOBP 8通过结合和转运植物挥发性物质发挥嗅觉功能。本研究为进一步了解C. suppressalis.
At the peripheral level of the insect olfaction, odorant binding proteins (OBPs) are thought to bind and transport exogenous hydrophobic volatiles to the odorant receptors (ORs) located on the dendrite membrane of the olfactory neurons. In this study, cDNA sequences of 29 OBP genes from Chilo suppressalis, a notorious rice pest, were identified, with 15 of them being newly reported. The tissue and temporal expression patterns of these CsupOBPs were determined by RT-PCR, revealing thatCsupOBP8,10and24were specifically expressed in the heads at larval stage and in antennae of both sexes at adult stage. In addition,CsupOBP 6,9and18were expressed in much higher levels in heads than abdomen at the larval stage, whileCsupPBP4andCsupOBP1,3,8,11and24were specifically expressed in antennae of both sexes at the adult stage. Such expression profile strongly suggests olfactory roles of these genes. Furthermore, the function of CsupOBP8 was chosen to be investigated by the competitive fluorescence binding assay. Results showed that CsupOBP8 displayed high binding affinities (Ki = 4.9–15.0 μM) with plant volatile β-ionone, nerolidol, farnesol and 2-hexanone, suggesting that CsupOBP8 plays olfactory roles through binding and transporting the plant volatiles. The study provides an important base for understanding the olfactory mechanisms inC. suppressalis.