Low expression of ferroxidases is implicated in the iron retention in human atherosclerotic plaques
Low expression of ferroxidases is implicated in the iron retention in human atherosclerotic plaques
复制标题
亚铁氧化酶的低表达与人类动脉粥样硬化斑块中的铁保留有关
DOI：
10.1016/j.bbrc.2015.07.091
复制
发表时间：
2015-09-04
影响因子：
3.1
通讯作者：
Qiao, Tong
中科院分区：
文献类型：
--
作者：
Ji, Jiajie;Zhou, Yu;Qiao, Tong
铁在动脉粥样硬化进展中的作用仍有争议。为探讨动脉粥样硬化斑块与铁代谢的关系及铁在斑块巨噬细胞中的蓄积情况，采用油红0染色法检测动脉粥样硬化斑块脂质，酶联免疫吸附法检测斑块内脂质（总胆固醇、游离胆固醇）和血清铁调素，Western-blot检测铁相关蛋白，免疫组织化学和免疫荧光测定来定位巨噬细胞中的膜铁转运蛋白1。测定血清铁和转铁蛋白饱和度。结果证实动脉粥样硬化斑块均为富脂斑块。与正常血管壁相比，动脉粥样硬化斑块中的铁蛋白和膜铁转运蛋白I水平显著升高。值得注意的是，我们发现斑块组织中的铁氧化酶铜蓝蛋白和铁蛋白水平比正常血管低得多，而血清铁调素、铁和转铁蛋白饱和度的含量在这两组中相似。这一新的发现表明，亚铁不能被氧化成三价铁可能是斑块中铁滞留的潜在机制。(C)2015 Elsevier Inc. All rights reserved.
The effect of iron on the progress of atherosclerosis is still controversial. To explore the relationship between atherosclerotic plaques and iron metabolism and how iron is accumulated in plaque macrophages, we performed Oil red 0 staining to detect the lipid of the atherosclerotic plaques, enzyme-linked immunosorbent assay to detect the intracellular lipids (total cholesterol, free cholesterol) and serum hepcidin, Western-blot to examine the iron-related proteins, immunohistochemical and immunofluorescence assays to localize ferroportin 1 in macrophages. The contents of serum iron and transferrin saturation were measured. The results confrimed that atherosclerotic plaques were all lipid-rich. Compared to normal vessel wall, atherosclerotic plaques had significantly higher levels of ferritin and ferroportin I. Strikingly, we found the much lower levels of ferroxidases ceruloplasmin and hephaestin in plaque tissue than the normal vessel, while the content of serum hepcidin, iron and transferrin saturation were similar in these two groups. The novel finding suggests that the inability of ferrous iron to be oxidized into ferric iron might be a potential mechanism for iron retention in plaques. (C) 2015 Elsevier Inc. All rights reserved.