Effect of transplantation with autologous bone marrow stem cells on acute myocardial infarction
Effect of transplantation with autologous bone marrow stem cells on acute myocardial infarction
复制标题
自体骨髓干细胞移植治疗急性心肌梗死的效果
DOI：
10.1016/j.ijcard.2011.05.077
复制
发表时间：
2013-01-20
影响因子：
3.5
通讯作者：
Chen, Lin
中科院分区：
文献类型：
--
作者：
Peng, Chaoquan;Yang, Ke;Chen, Lin
背景：探讨冠状动脉内移植自体间充质干细胞(MSCs)治疗猪心肌梗死(MI)的效果。方法：将携带绿色荧光蛋白(GFP)基因的慢病毒载体导入MSCs，体外用超顺磁性氧化铁(SPIO)标记。采用球囊结扎法建立急性心肌梗死(AMI)模型。实验组(n=5)使用OTW球囊装置经梗塞相关冠状动脉注入双标记MSC悬浮液，对照组(n=5)注射生理盐水。通过磁共振成像(MRI)、单光子发射计算机断层扫描(SPECT)和超声心动图(UCG)评价移植前和移植后8周干细胞迁移和心功能改善情况。移植后8周取心肌组织进行组织病理学分析。结果：骨髓间充质干细胞胞浆内可见蓝色SPIO颗粒。标记率达到100%。MSC移植后，MRI显示心肌梗死区聚集的SPIO标记的MSCs呈低信号。随着时间的推移，信号逐渐减弱，区域缩小。术后8周SPECT显示心肌充盈缺陷减少，存活心肌数量明显增加。移植后8周，超声心动图较对照组有明显改善。心肌梗死边缘带可见普鲁士蓝阳性细胞。结论：MRI可以实现对SPIO标记的MSCs的活体连续示踪。SPIO标记的MSCs冠脉内移植可提高心功能，促进心肌存活。(C)2011爱思唯尔爱尔兰有限公司。保留所有权利。
Background: This study was designed to investigate the effects of treatment with autologous mesenchymal stem cells (MSCs) by intracoronary transplantation on myocardial infarction (MI) in swine.Methods: MSCs were transfected with a lentiviral vector carrying the gene encoding green fluorescent protein (GFP) and labeled in vitro with superparamagnetic iron oxide (SPIO). An acute MI (AMI) model was established by percutaneous balloon occlusion. Dual-labeled MSC suspensions were injected through the infarct-related coronary artery using an over-the-wire (OTW) balloon device in the experimental group (n= 5), while normal saline was injected into the control (n= 5). Stem cell migration and improvements of cardiac function were evaluated by magnetic resonance imaging (MRI), single-photon emission computed tomography (SPECT), and ultrasonic cardiogram (UCG) both before MSC transplantation and after 8 weeks. At 8 weeks after transplantation, myocardial tissue was analyzed by histopathologic analyses.Results: Blue SPIO particles were in the cytoplasm of the MSCs. The labeling efficiency reached 100%. MRI revealed hypointensities of SPIO-labeled MSCs that were clustered in the myocardial infarct area after MSC transplantation. As time progressed, the signal gradually weakened and the area shrank. SPECT revealed that the myocardial filling defect was reduced and the amount of surviving myocardium was significantly increased at 8 weeks. UCG revealed significant improvement when compared with the control at 8 weeks after transplantation. Prussian blue-positive cells were observed in the MI border zone. Fluorescence-positive cells were also observed, but the number of such cells was fewer than before.Conclusions: In vivo serial tracking of SPIO-labeled MSCs can be achieved by MRI. Intracoronary transplantation of SPIO-labeled MSCs can increase cardiac function and promote myocardial viability. (C) 2011 Elsevier Ireland Ltd. All rights reserved.