CELL-SPECIFIC IMMUNOHISTOCHEMICAL LOCALIZATION OF A CELLULAR RETINOL-BINDING PROTEIN (TYPE-2) IN THE SMALL-INTESTINE OF RAT
CELL-SPECIFIC IMMUNOHISTOCHEMICAL LOCALIZATION OF A CELLULAR RETINOL-BINDING PROTEIN (TYPE-2) IN THE SMALL-INTESTINE OF RAT
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DOI：
10.1073/pnas.82.14.4707
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
ONG, DE
中科院分区：
文献类型：
--
作者：
CROW, JA;ONG, DE
一种新的视黄醇结合蛋白，这是明显不同于众所周知的细胞视黄醇结合蛋白，CRBP纯化。这种蛋白质被命名为细胞视黄醇结合蛋白II型[CRBP（II）]，几乎只在成年大鼠的小肠中发现，其水平比CRBP高1000倍。这2种蛋白质的细胞定位在大鼠小肠中进行了测定。使用免疫组织化学技术，小肠的吸收细胞，从十二指肠到回肠，强烈染色时，使用抗CRBP（II）的抗血清。更强烈的染色，观察到在吸收细胞附近的绒毛的顶端比那些位于绒毛的基部。Lieberkuhn隐窝中的增殖细胞仅轻度染色（如果有的话）。绒毛杯状细胞不染色。当检查包含胃十二指肠连接部的组织切片时，在胃上皮中未观察到染色，而十二指肠最近端部分的上皮显示出非常强的染色。在包含回盲部的组织切片中，染色在回肠远端末端突然终止。在结肠上皮中未观察到染色。CRBP在小肠中的细胞定位与CRBP（II）的细胞定位有很大不同。当使用亲和纯化的抗CRBP时，小肠上皮细胞未显示染色。在固有层和肠道相关淋巴组织内的细胞中观察到结缔组织细胞染色。这2种蛋白质的细胞特异性定位模式表明，CRBP（II），而不是CRBP，是在视黄醇的吸收中发挥作用的蛋白质。
A new retinol-binding protein that is distinctly different from the well-known cellular retinol-binding protein, CRBP was purified. This protein, which was named cellular retinol-binding protein type II [CRBP(II)], was found almost exclusively in the small intestine of the adult rat at levels 1000 times greater than that of CRBP. The cellular location of these 2 proteins was determined in the small intestine of the rat. Using an immunohistochemical technique, the absorptive cells of the small intestine, from the duodenum to the ileum, were strongly stained when antiserum against CRBP(II) was used. More intense staining was observed in absorptive cells near the tips of the villi than in those located at the base of the villi. The proliferative cells in the crypts of Lieberkuhn were stained only lightly if at all. Goblet cells in the villi did not stain. When tissue sections containing the gastroduodenal junction were examined, no staining was observed in the gastric epithelium, while the epithelium of the most proximal portion of the duodenum showed very strong staining. In tissue sections containing the ileocecal junction, staining terminated abruptly at the end of the distal ileum. No staining was observed in the epithelium of the colon. The cellular location of CRBP in the small intestine was quite different from the cellular location of CRBP(II). The epithelial cells of the small intestine showed no staining when affinity-purified anti-CRBP was used. Staining was observed for connective tissue cells in the lamina propria and in cells located within the gut-associated lymphoid tissue. The cell-specific localization pattern determined for these 2 proteins suggest that CRBP(II), rather than CRBP, is the protein that plays a role in the absorption of retinol.