Beta adrenergic receptors and cyclic AMP levels in intact human lymphocytes: effects of age and gender.
Beta adrenergic receptors and cyclic AMP levels in intact human lymphocytes: effects of age and gender.
复制标题
完整人淋巴细胞中的β肾上腺素能受体和环AMP水平：年龄和性别的影响。
DOI：
10.1016/0024-3205(84)90411-9
复制
发表时间：
1984
期刊：
影响因子：
6.1
通讯作者：
Golbourne,T
中科院分区：
文献类型：
--
作者：
Halper,JP;Mann,JJ;Weksler,ME;Bilezikian,JP;Sweeney,JA;Brown,RP;Golbourne,T
利用配体[125I]-iodocyanopindolol （[125I]ICYP）在完整的人外周血淋巴细胞上证实了一种饱和的、立体特异性的高亲和力β 2肾上腺素能受体。本文描述了一种平行测量饱和结合等温线和异丙肾上腺素- camp反应性的方法，该方法是从40毫升全血中分离的完整淋巴细胞的分裂样本。在健康受试者中，β受体密度（Bmax）与最大异丙肾上腺素生成cAMP与基础水平之比呈显著正相关（r=0.65, p < 0.001）。年龄与异丙肾上腺素诱导的基础cAMP水平的增加呈显著正相关。老年女性服用异丙肾上腺素后cAMP水平明显高于老年男性。这些影响很大程度上是由于老年受试者的cAMP基础水平较低。β受体结合指数（Bmax和KD）在男性和女性之间没有差异，也没有随年龄变化。年龄和性别对cAMP水平的影响似乎至少部分是由独立于受体的机制介导的。该方法描述了一种方便的平行测量小血液样本中β受体结合和cAMP水平的方法，代表了研究人类β受体功能的有用模型。
A saturable, stereospecific high affinity beta2adrenergic receptor was demonstrated on intact human peripheral blood lymphocytes using the ligand [125I]-iodocyanopindolol ([125I]ICYP). A method is described for parallel measurements of saturation binding isotherms and isoproterenol-cAMP responsiveness in split samples of intact lymphocytes isolated from 40 ml. of whole blood. A significant positive correlation between beta receptor density (Bmax) and the ratio of maximal isoproterenol-generated cAMP to basal levels was found in healthy subjects (r=0.65, p < 0.001). A significant positive correlation was found between age and the fold increase over basal cAMP levels induced by isoproterenol. Older females had a significantly higher fold increase in cAMP levels after isoproterenol than older males. These effects were largely accounted for by the lower basal levels of cAMP in older subjects. Beta receptor binding indices (Bmax and KD) did not differ between males and females, or change with aging. The effects of age and sex upon cAMP levels appear to be at least partly mediated by mechanisms independent of the beta receptor. The method, which describes a convenient assay for parallel measurement of beta receptor binding and cAMP levels in small blood samples, represents a useful model for studying human beta receptor function.