CHARACTERISTICS OF A HUMAN CELL LINE TRANSFORMED BY DNA FROM HUMAN ADENOVIRUS TYPE-5
CHARACTERISTICS OF A HUMAN CELL LINE TRANSFORMED BY DNA FROM HUMAN ADENOVIRUS TYPE-5
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DOI：
10.1099/0022-1317-36-1-59
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发表时间：
1977-01-01
影响因子：
3.8
通讯作者：
NAIRN, R
中科院分区：
文献类型：
--
作者：
GRAHAM, FL;SMILEY, J;NAIRN, R
通过将细胞暴露于5型腺病毒DNA的剪切片段来转化人胚肾细胞。转化的细胞（指定为293细胞）表现出许多转化的特征，包括病毒特异性肿瘤抗原的加工。通过35 S-蛋氨酸标记和SDS-PAGE [十二烷基硫酸钠-聚丙烯酰胺凝胶电泳]对293细胞中合成的多肽进行分析，结果显示合成模式可变，在许多方面与其他人类细胞中观察到的不同。在通过乳过氧化物酶催化的放射性碘标记细胞表面时，注意到没有类似于250 K [千道尔顿]（LETS [大，外部转化敏感]）糖蛋白的标记多肽。标记的细胞RNA与5型腺病毒DNA的Hind III限制性片段的杂交表明在常规的左手端有一部分腺病毒基因组的转录。
Human embryonic kidney cells were transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumor antigen. Analysis of the polypeptides synthesized in the 293 cells by labeling with 35S-methionine and SDS-PAGE [sodium dodecyl sulfate-polyacrylamide gel electrophoresis] showed a variable pattern of synthesis, different in a number of respects from that seen in other human cells. On labeling the surface of cells by lactoperoxidase catalyzed radio-iodination, the absence of a labeled polypeptide analogous to the 250 K [kilodalton] (LETS [large, external transformation sensitive]) glycoprotein was noted. Hybridization of labeled cellular RNA with Hind III restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.