Prss56 expression in the rodent hypothalamus: Inverse correlation with pro-opiomelanocortin suggests oscillatory gene expression in adult rat tanycytes.
Prss56 expression in the rodent hypothalamus: Inverse correlation with pro-opiomelanocortin suggests oscillatory gene expression in adult rat tanycytes.
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啮齿动物下丘脑中的 Prss56 表达：与阿片黑皮素原呈负相关，表明成年大鼠单细胞中存在振荡基因表达。
DOI：
10.1002/cne.24504
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Lechan,RonaldM
中科院分区：
文献类型：
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作者：
Wittmann,Gábor;Lechan,RonaldM
我们最近报道，表达前阿黑皮素（Pomc）的下丘脑伸长细胞的数量在成年大鼠的大脑中是高度可变的。虽然其原因和意义仍然未知，但识别伸长细胞中的其他表达基因可能有助于理解这一奇怪的现象。在这项原位杂交研究中，我们报告了Prss 56基因，它编码胰蛋白酶样丝氨酸蛋白酶，并在神经干/祖细胞中表达，在成年大鼠的伸长细胞中显示出类似的可变mRNA表达，并与伸长细胞PomcmRNA.Prss56在α1，β1，α2亚群和一些中隆起γ伸长细胞中表达，Prss 56也在腹内侧核和弓状核实质中的波形蛋白阳性延伸细胞样细胞以及垂体结节部的促甲状腺素β亚基表达细胞中表达。与成年人相比，Prss 56表达在青春期大鼠的伸长细胞中均匀地高。在小鼠中，还观察到表达Prss 56的伸长细胞和实质细胞，但数量较少且无显著变化。结果鉴定Prss 56是在成年大鼠的伸长细胞中表达的第二个基因。我们提出，变量，负相关的表达Prss 56和Pomcreflect周期性振荡的基因表达在伸长细胞，而不是稳定的表达水平，个体大鼠之间的变化。Prss 56与POMC之间可能存在功能联系，Prss 56可作为迁移性伸长细胞的标记物。
We recently reported that the number of hypothalamic tanycytes expressing pro‐opiomelanocortin (Pomc) is highly variable among brains of adult rats. While its cause and significance remain unknown, identifying other variably expressed genes in tanycytes may help understand this curious phenomenon. In thisin situhybridization study, we report that thePrss56gene, which encodes a trypsin‐like serine protease and is expressed in neural stem/progenitor cells, shows a similarly variable mRNA expression in tanycytes of adult rats and correlates inversely with tanycytePomcmRNA.Prss56was expressed in α1, β1, subsets of α2, and some median eminence γ tanycytes, but virtually absent from β2 tanycytes.Prss56was also expressed in vimentin positive tanycyte‐like cells in the parenchyma of the ventromedial and arcuate nuclei, and in thyrotropin beta subunit‐expressing cells of the pars tuberalis of the pituitary. In contrast to adults,Prss56expression was uniformly high in tanycytes in adolescent rats. In mice,Prss56‐expressing tanycytes and parenchymal cells were also observed but fewer in number and without significant variations. The results identifyPrss56as a second gene that is expressed variably in tanycytes of adult rats. We propose that the variable, inversely correlating expression ofPrss56andPomcreflect periodically oscillating gene expression in tanycytes rather than stable expression levels that vary between individual rats. A possible functional link between Prss56 and POMC, andPrss56as a potential marker for migrating tanycytes are discussed.