Biomechanical changes in oxazolone-induced colitis in BALB/C mice.
Biomechanical changes in oxazolone-induced colitis in BALB/C mice.
复制标题
BALB/C 小鼠恶唑酮诱导的结肠炎的生物力学变化。
DOI：
10.1016/j.jbiomech.2009.01.028
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发表时间：
2009
影响因子：
2.4
通讯作者：
Gregersen,Hans
中科院分区：
文献类型：
--
作者：
Yang,Jian;Zhao,Jingbo;Nakaguchi,Toshiya;Gregersen,Hans
溃疡性结肠炎（UC）与肠道和肠道外的临床表现有关。UC的深刻有机变化表明结肠机械和机械感觉功能受到影响。目的是研究恶唑酮诱导的BALB/C小鼠UC的远端结肠的急性形态学和生物力学特性。研究了6只正常雄性BALB/C小鼠和10只恶唑酮诱导的UC小鼠。UC是由表皮和直肠内给药恶唑酮引起的。机械测试作为膨胀实验进行，结肠膨胀至20cmH2O。同时记录压力、外径和长度。计算了环向和纵向应力和应变。对肠道标本进行组织学处理。急性和慢性炎症细胞浸润粘膜。观察到粘膜出血，不规则溃疡，隐窝脓肿和上皮边界破坏。溃疡内粘膜较正常对照组薄，但溃疡周围粘膜及粘膜下层较正常对照组厚（P<0.05）。恶唑酮诱导的结肠炎使肠壁周长和肠壁截面积增加（P<0.01），浆膜开口角和残余应变增加（P<0.01）。此外，周向和纵向刚度在UC壁面增加，并在纵向上最明显。打开角度和残余应变与壁厚、面积和炎症程度呈线性相关。综上所述，在UC的发展过程中，结肠的形态和生物力学发生了变化。僵硬度的增加可能导致UC患者的功能异常。
Ulcerative colitis (UC) is associated with intestinal and extra intestinal clinical manifestations. The profound organic changes in UC indicate that the colonic mechanical and mechanosensory functions are affected. The aim was to study acute morphological and biomechanical properties of the distal colon in oxazolone-induced UC in BALB/C mice. Six normal male BALB/C mice and 10 oxazolone-induced UC mice were studied. UC was induced by epicutaneous and intrarectal administration of oxazolone. The mechanical test was done as a distension experiment where the colon was distended up to 20cmH2O. The pressure, outer diameter and length were recorded simultaneously. Circumferential and longitudinal stresses and strains were computed. The intestinal specimens were processed for histology. The mucosa was infiltrated with acute and chronic inflammatory cells. Mucosal bleeding, irregular ulcers crypt abscess, and destruction of the epithelial border were observed. Although, the mucosa in ulcers was much thinner than in the normal controls, the mucosa and submucosa around the ulcer were thicker than in the normal controls (P<0.05). Oxazolone-induced colitis increased the circumferences and wall cross-sectional area (P<0.01), the opening angle and residual strain at the serosa increased (P<0.01). Furthermore, the circumferential and longitudinal stiffness increased in the UC wall and was most pronounced in longitudinal direction. The opening angle and residual strain was linearly correlated to the wall thickness, area and inflammation degree. In conclusion, morphological and biomechanical changes of the colon occurred during the development of UC. The increased stiffness may contribute to the abnormal function in patients with UC.