Detoxification of nitric oxide by the flavorubredoxin of Salmonella enterica serovar Typhimurium.
Detoxification of nitric oxide by the flavorubredoxin of Salmonella enterica serovar Typhimurium.
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鼠伤寒沙门氏菌的风味素对一氧化氮的解毒作用。
DOI：
10.1042/bst0330198
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发表时间：
2005
影响因子：
3.9
通讯作者：
Stephen Spiro
中科院分区：
文献类型：
--
作者：
Paul C. Mills;David J. Richardson;Jay C. D. Hinton;Stephen Spiro
沙门氏菌拥有多种酶，利用NO作为底物，因此可能有助于机体抵抗巨噬细胞的亚硝化杀伤能力。Flavorubredoxin是一种对氧敏感的酶，可以将NO还原为一氧化二氮。对编码flavorubredoxin的肠沙门氏菌血清型鼠伤寒杆菌norV基因进行了破坏，并测定了突变体对NO的敏感性。norV突变体对NO的敏感性高于野生型鼠伤寒沙门氏菌，但在短暂抑制后恢复了生长。突变表型表明鼠伤寒沙门氏菌在厌氧条件下使用多种酶来解毒NO，其中一种是flavorubredoxin。
Salmonella possesses multiple enzymes that utilize NO as a substrate, and could therefore contribute to the organism's ability to resist nitrosative killing by macrophages. Flavorubredoxin is an oxygen-sensitive enzyme that reduces NO to nitrous oxide. The Salmonella enterica serovar Typhimurium norV gene encoding flavorubredoxin was disrupted and the NO sensitivity of the mutant was determined. The norV mutant showed a greater sensitivity to NO than wild-type S. Typhimurium, but did recover growth after a transient inhibition. The mutant phenotype suggests that multiple enzymes are employed by S. Typhimurium to detoxify NO under anaerobic conditions, one of which is flavorubredoxin.