Detection of anti-CUEC-23 antibodies in serum of patients with esophageal squamous cell carcinoma: a possible new serum marker for esophageal cancer
Detection of anti-CUEC-23 antibodies in serum of patients with esophageal squamous cell carcinoma: a possible new serum marker for esophageal cancer
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DOI：
10.1007/s00535-009-0060-8
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发表时间：
2009-07-01
影响因子：
6.3
通讯作者：
Hiwasa, Takaki
中科院分区：
文献类型：
--
作者：
Shimada, Hideaki;Kagaya, Akiko;Hiwasa, Takaki
基于重组cDNA表达克隆的抗原血清学鉴定（SEREX）是检测新的肿瘤特异性抗原的一种成熟方法。SEREX抗原抗体可用于食管鳞状细胞癌（SCC）的检测。方法利用食管鳞状细胞癌患者血清筛选人食管鳞状细胞癌细胞系噬菌体cDNA文库。采用纯化的重组抗原蛋白，分别采用Western blotting和酶联免疫吸附试验（ELISA）检测血清SEREX抗原抗体的存在和水平。结果新鉴定的食管鳞状细胞癌抗原由位于1号染色体上的新基因CUEC-23编码。54例食管鳞状细胞癌患者中有14例（26%）血清检测到cuec -23抗体（s-CUEC-23-Abs）。食管SCCs中s-CUEC-23-Abs、CEA、SCC-Ag和CYFRA21-1阳性分别为44.4%、41%和52%。s-CUEC-23-Abs的存在与临床病理变量之间没有可检测到的关联。ELISA结果显示，食管SCC患者血清s-CUEC-23-Abs水平明显高于健康志愿者（以健康对照组s-CUEC-23-Abs平均+ 3 SD为截止值，前者为17%）。结论经SEREX筛选，发现一种新的肿瘤抗原CUEC-23。s-CUEC-23-Abs可能是检测食管鳞状细胞癌的有效血清标志物。
Background Serological identification of antigens by recombinant cDNA expression cloning (SEREX) is an established method for detecting new tumor-specific antigens. Antibodies to SEREX antigens may be useful for the detection of esophageal squamous cell carcinoma (SCC).Methods A phage cDNA library of a human esophageal SCC cell line was screened using sera of patients with esophageal SCC. The presence and levels of serum antibodies to SEREX antigens were established by Western blotting and enzyme-linked immunosorbent assay (ELISA) using purified recombinant antigen proteins, respectively.Results The newly identified esophageal SCC antigen is encoded by a novel gene located on chromosome 1, here designated CUEC-23. SerumCUEC-23-antibodies (s-CUEC-23-Abs) were detected in 14 of 54 patients with esophageal SCC (26%) by Western blot analysis. Esophageal SCCs were positive for s-CUEC-23-Abs together with CEA, SCC-Ag or CYFRA21-1 in 44, 41 and 52% of cases, respectively. There was no detectable association between the presence of s-CUEC-23-Abs and clinicopathological variables. ELISA showed that the levels of s-CUEC-23-Abs were significantly higher in patients with esophageal SCC than in healthy volunteers (17% in the former using the mean + 3 SD of s-CUEC-23- Abs in healthy controls as the cutoff).Conclusion A new tumor antigen, CUEC-23, was identified by SEREX screening. s-CUEC-23-Abs might be a useful serum marker to detect esophageal SCC.