Proteomic Lung Analysis of Mice with Ventilator-Induced Lung Injury (VILI) Using iTRAQ-Based Quantitative Proteomics
Proteomic Lung Analysis of Mice with Ventilator-Induced Lung Injury (VILI) Using iTRAQ-Based Quantitative Proteomics
复制标题
使用基于 iTRAQ 的定量蛋白质组学对呼吸机引起的肺损伤 (VILI) 小鼠进行蛋白质组肺分析
DOI：
10.1248/cpb.c17-00844
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发表时间：
2018-07-01
影响因子：
1.7
通讯作者：
Deng, Xiaoming
中科院分区：
文献类型：
--
作者：
Ding, Haoshu;Wang, Yan;Deng, Xiaoming
Ventilator-induced lung injury (VILI) has implications for mortality from acute lung injury (ALI) and for acute respiratory distress syndrome (ARDS) patients; the complicated mechanisms of VILI have not been well defined. To discover new biomarkers and mechanisms of VILI, isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-based quantitative proteomics were applied to identify differentially expressed proteins in mice treated with high tidal volume ventilation (HV), low tidal volume ventilation (LV) and lipopolysaccharide (LPS). A total of 14 dysregulated proteins showed the same change trend both in the LV and HV group and no change in the LPS group, and most importantly, the fold change of these proteins increased with the increase of volume ventilation, which indicates these proteins may be considered as potential markers specific for VILI. Ingenuity pathway analysis (IPA) canonical pathways analysis identified the top 4 canonical pathways, including the extrinsic prothrombin activation pathway, coagulation systems, the intrinsic prothrombin activation pathway and the acute phase response, suggesting that these pathways, as associated with these proteins' expression, may be important therapeutic targets for reducing VILI. These findings will provide a new perspective for understanding the pathogenesis of VILI in the future.
