Complete Nucleotide Sequence of a Strawberry Isolate of Beet Pseudoyellows Virus
Complete Nucleotide Sequence of a Strawberry Isolate of Beet Pseudoyellows Virus
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DOI：
10.1023/b:viru.0000025771.48128.f8
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发表时间：
2004-04
期刊：
影响因子：
1.6
通讯作者：
I. Tzanetakis;R. Martin
中科院分区：
文献类型：
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作者：
I. Tzanetakis;R. Martin
在我们鉴定草莓苍白球病病原体的工作中，我们发现了一株苍白球病阳性的植物，该植物在使用代表草莓苍白球病相关病毒（SPaV）基因组多个区域的引物组进行RT-PCR后没有产生任何扩增子，并且未能与针对SPaV重组外壳蛋白（CP）的抗体反应。从该植株中提取的双链RNA显示出与SPaV相似的模式，表明另一种蔓状病毒可能感染草莓。对该病毒的热激70同源基因（HSP 70 h）的多个cDNA克隆进行序列分析，结果表明该病毒为甜菜假黄化病毒（BPYV）。对BPYV-草莓全核苷酸序列的分析表明，该分离物与黄瓜黄化病毒（CuYV）（BPYV的黄瓜株）相比具有几个明显的特征，包括CuYV中未发现的完整ORF。
In our effort to identify the causal agent(s) of strawberry pallidosis we found a single pallidosis positive plant that did not give any amplicons after RT-PCR using primer sets representing multiple regions ofStrawberry pallidosis associated virus(SPaV) genome and failed to react with antibodies directed against the recombinant coat protein (CP) of SPaV. DsRNA extracted from this plant showed a similar pattern to that of SPaV indicating that another crinivirus may infect strawberry. Sequence analysis of multiple cDNA clones corresponding to the heat shock 70 homolog gene (HSP70h) of the unknown virus indicated that it wasBeet pseudoyellows virus(BPYV). Analysis of the complete nucleo- tide sequence of BPYV-strawberry revealed that this isolate has several distinct features when compared toCucumber yellows virus(CuYV), a cucumber strain of BPYV, including an entire ORF not found in CuYV.