A novel Meloidogyne graminicola effector, MgMO237, interacts with multiple host defence-related proteins to manipulate plant basal immunity and promote parasitism
A novel Meloidogyne graminicola effector, MgMO237, interacts with multiple host defence-related proteins to manipulate plant basal immunity and promote parasitism
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一种新型的禾本科根结线虫效应子 MgMO237，与多种宿主防御相关蛋白相互作用，操纵植物基础免疫并促进寄生
DOI：
10.1111/mpp.12671
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发表时间：
2018-08-01
影响因子：
4.9
通讯作者：
Liao, Jinling
中科院分区：
文献类型：
--
作者：
Chen, Jiansong;Hu, Lili;Liao, Jinling
植物寄生线虫可以将效应蛋白分泌到宿主组织中，以促进它们的寄生。在这项研究中，我们报道了一种新的效应蛋白，mGMO237，它只在食道腺背部细胞中表达，并在寄生的第三/第四期禾谷分线虫幼虫中显著上调。在水稻根部原生质体中瞬时表达的结果表明，该基因定位于寄主细胞的胞质和胞核。过量表达MgMO237的水稻植株对稻瘟病菌的感受性增加。相反，表达针对镁MO237的RNA干扰载体的水稻植株对稻瘟病菌表现出更强的抗性。此外，酵母双杂交和免疫共沉淀实验表明，该基因与水稻内源蛋白1，3-β-葡聚糖合成酶组分(OsGSC)、富含半胱氨酸重复序列的分泌蛋白55(OsCRRSP55)和病程相关的BetvI家族蛋白(OsBetvI)具有特异性相互作用，这些蛋白均与寄主防御相关。此外，MgMO237还可以抑制寄主防御反应，包括寄主防御相关基因的表达、细胞壁痂蛋白的沉积和活性氧的爆发。这些结果表明，在线虫寄生后期，效应子MgMO237可能通过与多种寄主防御相关蛋白相互作用，抑制植物的基础免疫，从而促进稻瘟病菌的寄生。
Plant-parasitic nematodes can secrete effector proteins into the host tissue to facilitate their parasitism. In this study, we report a novel effector protein, MgMO237, from Meloidogyne graminicola, which is exclusively expressed within the dorsal oesophageal gland cell and markedly up-regulated in parasitic third-/fourth-stage juveniles of M.graminicola. Transient expression of MgMO237 in protoplasts from rice roots showed that MgMO237 was localized in the cytoplasm and nucleus of the host cells. Rice plants overexpressing MgMO237 showed an increased susceptibility to M.graminicola. In contrast, rice plants expressing RNA interference vectors targeting MgMO237 showed an increased resistance to M.graminicola. In addition, yeast two-hybrid and co-immunoprecipitation assays showed that MgMO237 interacted specifically with three rice endogenous proteins, i.e. 1,3-beta-glucan synthase component (OsGSC), cysteine-rich repeat secretory protein 55 (OsCRRSP55) and pathogenesis-related BetvI family protein (OsBetvI), which are all related to host defences. Moreover, MgMO237 can suppress host defence responses, including the expression of host defence-related genes, cell wall callose deposition and the burst of reactive oxygen species. These results demonstrate that the effector MgMO237 probably promotes the parasitism of M.graminicola by interacting with multiple host defence-related proteins and suppressing plant basal immunity in the later parasitic stages of nematodes.