NEURONAL CHANGES IN THE SUBSTANTIA-NIGRA WITH AGING - A GOLGI-STUDY
NEURONAL CHANGES IN THE SUBSTANTIA-NIGRA WITH AGING - A GOLGI-STUDY
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DOI：
10.1097/00005072-199501000-00009
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发表时间：
1995-01-01
影响因子：
3.2
通讯作者：
TOLOSA, E
中科院分区：
文献类型：
--
作者：
CRUZSANCHEZ, FF;CARDOZO, A;TOLOSA, E
为了认识到黑质神经元的变化发生在老化，20人对照大脑13名男性和7名女性，平均年龄为61岁（范围20至93岁），没有神经系统疾病进行了检查使用高尔基体方法。树突和树突棘的定量研究，以及所获得的数据进行统计分析。对浸渍材料的平行切片进行组织学和免疫组织学研究，旨在识别可能的神经元细胞骨架异常。结果进行了比较，在其他条件下，如帕金森氏病（PD）和甲基-4-苯基吡啶（MPTP）的实验毒性黑质的变化。观察到三种不同的黑质神经元类型。衰老过程中的形态学变化包括胞体轮廓变形和树突状分支肿胀和串珠。在神经元类型中观察到的变化的定量评估显示树突和树突棘的显着损失，特别是在最古老的情况下。这些发现与以前在衰老过程中其他大脑区域的研究结果相似，但可以观察到最大的黑质神经元类型的特定脆弱性。树突的结瘤和串珠状方面让人想起先前在MPTP毒性中描述的那些变化。在最古老的病例中发现的树突静脉曲张也在PD的大黑质神经元树突中发现。细胞骨架异常已在PD中描述，但在本研究中未发现。因此，其他的病理生理机制不同的细胞骨架妥协发生在一些神经退行性疾病应该参与老化。
In order to recognize substantia nigra neuronal changes occurring in aging, 20 human control brains from 13 males and 7 females with a mean age of 61 years (range 20 to 93 years) without neurological disease were examined using the Golgi method. A quantitative study of dendrites and dendritic spines was performed as well as a statistical analysis of obtained data. Parallel sections to the impregnated material were histologically and immunohistologically studied with the aim to identify possible neuronal cytoskeletal abnormalities. Results were compared to changes of substantia nigra reported in other conditions such as Parkinson's disease (PD) and methyl-4-phenylpyridine (MPTP) experimental toxicity. Three different substantia nigra neuronal types were observed. Morphological changes during aging consisted of distorted profile of the cell body and swelling and beading of dendritic branches. The quantitative assessment of changes observed in neuronal types showed a significant loss of dendrites and dendritic spines, especially in the oldest cases. These findings were similar to those previously described in other cerebral areas during aging, but a specific vulnerability of the largest substantia nigra neuronal type could be observed. Nodulations and beaded aspects of dendrites are reminiscent of those changes previously described in MPTP toxicity. Dendritic varicosities found in the oldest cases have also been found in dendrites of large substantia nigra neurons in PD. Cytoskeletal abnormalities have been described in PD but were not found in the present study. Therefore, other pathophysiological mechanisms different from the cytoskeletal compromise occurring in some neurodegenerative diseases should be involved in aging.