The solution structure of the outer membrane lipoprotein OmlA from Xanthomonas axonopodis pv. citri reveals a protein fold implicated in protein–protein interaction
The solution structure of the outer membrane lipoprotein OmlA from Xanthomonas axonopodis pv. citri reveals a protein fold implicated in protein–protein interaction
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柑橘黄单胞菌外膜脂蛋白 OmlA 的溶液结构揭示了与蛋白质-蛋白质相互作用有关的蛋白质折叠。
DOI：
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
C. Benedetti
中科院分区：
文献类型：
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作者：
M. M. T. Vanini;A. Spisni;M. Sforça;T. Pertinhez;C. Benedetti
外膜脂蛋白 A (OmlA) 属于细菌小脂蛋白家族，广泛分布于 β 和 γ 变形菌中。尽管许多细菌脂蛋白的作用是已知的，但 OmlA 的生物学功能仍然难以捉摸。我们发现，在柑橘溃疡病病原体中，Xanthomonas axonopodis pv.在柑橘 (X. citri) 中，OmlA 与铁吸收调节剂 (Fur) 共同调节，当 X. citri 生长在柑橘叶上时，OmlA 的表达会增强，表明这些蛋白参与植物与病原体的相互作用。为了深入了解 OmlA 的功能，通过核磁共振确定了其构象和动态特征。该蛋白质具有高度灵活的 N 端和 C 端以及结构明确的核心，该核心由三个 β 链和两个小 α 螺旋组成，它们相互挤压形成双层 α/β 支架。这种蛋白质折叠类似于 β-内酰胺酶抑制蛋白 BLIP 的结构域，参与蛋白质-蛋白质结合。总之，OmlA 的结构确实表明该蛋白质可能与 X. citri 感染过程中所需的蛋白质-蛋白质相互作用有关。蛋白质 2008。© 2008 Wiley-Liss, Inc.
The outer membrane lipoprotein A (OmlA) belongs to a family of bacterial small lipoproteins widely distributed across the beta and gamma proteobacteria. Although the role of numerous bacterial lipoproteins is known, the biological function of OmlA remains elusive. We found that in the citrus canker pathogen, Xanthomonas axonopodis pv. citri (X. citri), OmlA is coregulated with the ferric uptake regulator (Fur) and their expression is enhanced when X. citri is grown on citrus leaves, suggesting that these proteins are involved in plant‐pathogen interaction. To gain insights into the function of OmlA, its conformational and dynamic features were determined by nuclear magnetic resonance. The protein has highly flexible N‐ and C‐ termini and a structurally well defined core composed of three β‐strands and two small α‐helices, which pack against each other forming a two‐layer alpha/beta scaffold. This protein fold resembles the domains of the β‐lactamase inhibitory protein BLIP, involved in protein–protein binding. In conclusion, the structure of OmlA does suggest that this protein may be implicated in protein–protein interactions required during X. citri infection. Proteins 2008. © 2008 Wiley‐Liss, Inc.
影响因子：
2.9
作者：
Lefevre, JF;Dayie, KT;Wagner, G
通讯作者：
Wagner, G
影响因子：
2.9
作者：
Parsons, LM;Lin, F;Orban, J
通讯作者：
Orban, J