QTL mapping of aroma compounds analysed by headspace solid-phase microextraction gas chromatography in the apple progeny 'Discovery' x 'Prima'
QTL mapping of aroma compounds analysed by headspace solid-phase microextraction gas chromatography in the apple progeny 'Discovery' x 'Prima'
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DOI：
10.1007/s11032-008-9252-9
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发表时间：
2009-04-01
影响因子：
3.1
通讯作者：
Weber, W. E.
中科院分区：
文献类型：
--
作者：
Dunemann, F.;Ulrich, D.;Weber, W. E.
改良苹果品种的果实品质是一个重要而复杂的育种目标。风味是苹果果实品质的关键因素之一，但尽管对挥发性物质的分析和生物化学知识知之甚少，苹果香气的遗传和分子基础。本研究的目的是使用苹果的饱和分子连锁图来确定诸如醇类、酯类和萜烯类的香气化合物的QTL，以及一些未鉴定的挥发性化合物的QTL（非靶向分析方法）。以AFLP和SSR标记为主要标记，构建了“发现”和“普瑞玛”两个苹果品种的亲本遗传图谱。'发现'和'Prima'表现出非常不同的挥发性模式，和'发现'大多具有较高的挥发性浓度相比，Vf-黑星病抗性'Prima'，它有它的起源于小果苹果种多花海棠。利用作图群体“C3”的150个F-1代个体的基因型数据和顶空固相微萃取气相色谱法获得的表型数据，通过区间作图法检测到了27个苹果果实挥发性物质的约50个QTL。在苹果17条染色体中，有12条染色体上检测到与苹果香气相关的挥发性物质的QTL，但分布不均匀。QTL主要聚集在连锁群LG 2、3和9上。在第一次尝试中，LOX（脂氧合酶）的候选基因，参与挥发性物质代谢的pueroxin，被定位在LG 9上，与酯型挥发物的QTL簇遗传相关。讨论了苹果香气育种的意义。
Improving fruit quality of apple varieties is an important but complex breeding goal. Flavour is among the key factors of apple fruit quality but in spite of the analytical and biochemical knowledge about volatiles little is known about the genetic and molecular bases of apple aroma. The aim of this study was to use a saturated molecular linkage map of apple to identify QTLs for aroma compounds such as alcohols, esters and terpenes, but also for a number of unidentified volatile compounds (non-targeted analysis approach). Two parental genetic maps were constructed for the apple cultivars 'Discovery' and 'Prima' by using mainly AFLP and SSR markers. 'Discovery' and 'Prima' showed very different volatile patterns, and 'Discovery' mostly had the higher volatile concentrations in comparison with the Vf-scab resistant 'Prima' which has its origin in the small-fruited apple species Malus floribunda. About 50 putative QTLs for a total of 27 different apple fruit volatiles were detected through interval mapping by using genotypic data of 150 F-1 individuals of the mapping population 'C3' together with phenotypic data obtained by head-space solid phase microextraction gas chromatography. QTLs for volatile compounds putatively involved in apple aroma were found on 12 out of the 17 apple chromosomes, but they were not evenly dispersed. QTLs were mainly clustered on linkage groups LG 2, 3 and 9. In a first attempt, a LOX (lipoxygenase) candidate gene, putatively involved in volatile metabolism, was mapped on LG 9, genetically associated with a cluster of QTLs for ester-type volatiles. Implications for aroma breeding in apple are discussed.