Isogenic auxotrophic mutant strains in the Aspergillus fumigatus genome reference strain AF293
Isogenic auxotrophic mutant strains in the Aspergillus fumigatus genome reference strain AF293
复制标题
DOI：
10.1007/s00203-004-0707-z
复制
发表时间：
2004-09
影响因子：
2.8
通讯作者：
Tao Xue;C. Nguyen;A. Romans;D. Kontoyiannis;G. May
中科院分区：
文献类型：
--
作者：
Tao Xue;C. Nguyen;A. Romans;D. Kontoyiannis;G. May
烟曲霉是一种普遍存在的真菌，是免疫抑制患者中常见的机会致病菌。由于其作为病原体的作用，它是相当大的实验兴趣。在A.烟曲霉基因组参考菌株AF293已被开发。利用分子遗传学方法，通过缺失argBandlysB分别获得精氨酸和赖氨酸营养缺陷型。分别用携带argBorlysB的质粒转化这些营养缺陷型菌株，导致在这些位点的有效整合。最后，这些菌株能够形成稳定的二倍体，这将进一步促进该真菌中基因功能的分析。此外，在AF293背景下开发这种营养缺陷型菌株的同基因组将使研究人员能够更方便地研究这种重要的机会性人类病原体。
Aspergillus fumigatusis a ubiquitous fungus that is a frequent opportunistic pathogen in immunosuppressed patients. Because of its role as a pathogen, it is of considerable experimental interest. A set of auxotrophic isogenic strains in theA. fumigatusgenome reference strain AF293 has been developed. Using molecular genetic methods, arginine and lysine auxotrophs were made by deletion ofargBandlysB, respectively. Transformation of these auxotrophic strains with plasmids carryingargBorlysB, respectively, results in efficient integration at these loci. Finally, these strains are able to form stable diploids, which should further facilitate analysis of gene functions in this fungus. Furthermore, the development of this isogenic set of auxotrophic strains in the AF293 background will enable investigators to study this important opportunistic human pathogen with greater facility.