A gene‐specific promoter element is required for optimal expression of the histone H1 gene in S‐phase.
A gene‐specific promoter element is required for optimal expression of the histone H1 gene in S‐phase.
复制标题
S 期组蛋白 H1 基因的最佳表达需要基因特异性启动子元件。
DOI：
10.1002/j.1460-2075.1988.tb02782.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
J. Wells
中科院分区：
文献类型：
--
作者：
S. Dalton;J. Wells
通过对导入HeLa细胞的组蛋白基因转录产物的分析表明，H1基因特异性元件(H1-box，5‘-AAACACA-3’)在体内调节该基因的S期表达。该元件的缺失或碱基替换会导致随机生长细胞中稳定状态的H1mRNA水平下降15-30倍，并消除同步细胞中细胞周期对转录的控制。H1特异元件内的突变取消了S转录的阶段控制，也消除了体外能够与该区域特异结合的序列特异核因子的结合。将多个拷贝的H1-box元件转染到细胞中，大大降低了H1 mRNA的水平，模拟了通过缺失或替换突变使基序失去功能时观察到的效果。相反，将突变的H1元素导入细胞没有可检测到的效果。综上所述，这些结果表明，H1-box和序列特异性反式作用因子之间的相互作用调节了体内H1基因的转录控制。
An H1 gene‐specific element (H1‐box, 5′‐AAACACA‐3′) modulates S‐phase expression of the gene in vivo as judged by analysis of transcripts from histone genes transfected into HeLa cells. Deletion or base‐substitution of the element causes a 15‐ to 30‐fold decrease in steady‐state H1 mRNA levels in randomly growing cells and eliminates cell cycle control of transcription in synchronized cells. Mutations within the H1‐specific element which abolish S‐phase control of transcription also eliminate binding of a sequence‐specific nuclear factor capable of binding specifically to this region in vitro. Transfection of multiple copies of H1‐box elements into cells drastically decreases H1 mRNA levels, mimicking the effect observed when the motif is rendered non‐functional by deletion or substitution mutagenesis. In contrast, introduction of mutated H1 elements into cells has no detectable effect. Together, these results imply that an interaction between the H1‐box and a sequence‐specific trans‐acting factor modulates transcriptional control of H1 genes in vivo.