Colorimetric Detection of Dopamine with J-Aggregate Nanotube-Integrated Hydrogel Thin Films
Colorimetric Detection of Dopamine with J-Aggregate Nanotube-Integrated Hydrogel Thin Films
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DOI：
10.1021/acsomega.0c01803
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发表时间：
2020-07
期刊：
影响因子：
4.1
通讯作者：
N. Reddy;Samuel Rhodes;Jiyu Fang
中科院分区：
文献类型：
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作者：
N. Reddy;Samuel Rhodes;Jiyu Fang
多巴胺 (DA) 缺乏在临床上与多种神经系统疾病有关。尿液 DA 的检测为诊断这些疾病和监测治疗提供了一种无创方法。在本文中，我们报道了石胆酸（LCA）和3,3'-二乙基硫二碳花青碘化物（DiSC2(5)）以等摩尔比在氨溶液中共组装成J-聚集体纳米管。通过将 J-聚集纳米管集成到石英比色皿壁上形成的透明琼脂糖水凝胶膜中，我们制造了一个便携式且可重复的传感器平台，用于光学检测合成尿液中的 DA。发现集成的 J-聚集体纳米管的 J-带强度随着 DA 浓度从 10 nM 增加到 80 nM 而线性下降，检测限为 ∼7 nM。检测机制基于从激发的 J-聚集体纳米管到吸附的 DA-醌的光致电子转移 (PET)。传感器平台采用的PET工艺可以减少抗坏血酸和尿酸对合成尿液中DA检测的干扰。 J-聚集体纳米管的离域激子贡献了传感器平台的高灵敏度。
The deficiency of dopamine (DA) is clinically linked to several neurological diseases. The detection of urinary DA provides a noninvasive method for diagnosing these diseases and monitoring therapies. In this paper, we report the coassembly of lithocholic acid (LCA) and 3,3′-diethythiadicarbocyanine iodide (DiSC2(5)) at the equimolar ratio in ammonia solution into J-aggregate nanotubes. By integrating the J-aggregate nanotubes into transparent agarose hydrogel films formed on the wall of quartz cuvettes, we fabricate a portable and reproducible sensor platform for the optical detection of DA in synthetic urine. The J-band intensity of the integrated J-aggregate nanotubes is found to linearly decrease with the increase of DA concentrations from 10 to 80 nM, giving the limit of detection of ∼7 nM. The detection mechanism is based on the photoinduced electron transfer (PET) from the excited J-aggregate nanotubes to adsorbed DA-quinone. The PET process used in the sensor platform can reduce the interference of ascorbic acid and uric acid in the detection of DA in synthetic urine. The high sensitivity of the sensor platform is contributed by the delocalized exciton of J-aggregate nanotubes.