Association of CiaRH with resistance of Streptococcus mutans to antimicrobial peptides in biofilms.
Association of CiaRH with resistance of Streptococcus mutans to antimicrobial peptides in biofilms.
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DOI：
10.1111/j.2041-1014.2012.00637.x
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发表时间：
2012-04
影响因子：
3.7
通讯作者：
Y. Mazda;M. Kawada-Matsuo;K. Kanbara;Y. Oogai;Y. Shibata;Y. Yamashita;S. Miyawaki;H. Komatsuzawa
中科院分区：
文献类型：
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作者：
Y. Mazda;M. Kawada-Matsuo;K. Kanbara;Y. Oogai;Y. Shibata;Y. Yamashita;S. Miyawaki;H. Komatsuzawa
变形链球菌是人类的致龋病原体。在口腔中持续存在，S。变形杆菌对来自宿主的几种抗菌因子具有抗性。在这项研究中，我们研究了对阳离子抗菌肽（AMP）的耐药机制，这是人类的先天免疫因子。因为dltA-D（磷壁酸生物合成）被报道影响其他细菌物种对AMP的敏感性，我们评估了S.变异体对AMP人β-防御素-I（hBD 1）、hBD 2、hBD 3和LL 37的作用。dltC突变体表现出对AMP的敏感性显著增加。dltC表达的调节涉及CiaRH，一个双组分系统。在野生型菌株中dltC的表达在生物膜细胞中比在增殖细胞中显著增加，而在ciaRH敲除突变体中表达没有增加。在生物膜细胞中，我们发现与野生型相比，ciaRH突变体对LL 37的易感性增加。结果表明，Dlt与S.在生物膜细胞中，变异株与AMP的结合受到CiaRH的调节。
Streptococcus mutans is a cariogenic pathogen in humans. To persist in the oral cavity, S. mutans is resistant against several antibacterial factors derived from the host. In this study, we investigated the mechanism of resistance to cationic antimicrobial peptides (AMPs), which are innate immune factors in humans. Because dltA-D (teichoic acid biosynthesis) was reported to affect the susceptibility to AMPs in other bacterial species, we evaluated the susceptibility of a dltC knockout mutant of S. mutans to the AMPs human beta-defensin-1 (hBD1), hBD2, hBD3 and LL37. The dltC mutant exhibited significantly increased susceptibility to AMPs. Regulation of dltC expression involved CiaRH, a two-component system. Expression of dltC in the wild-type strain was significantly increased in biofilm cells compared with that in planktonic cells, whereas expression was not increased in a ciaRH knockout mutant. In biofilm cells, we found that susceptibility to LL37 was increased in the ciaRH mutant compared with that in the wild type. From these results, it is concluded that Dlt is involved in the susceptibility of S. mutans to AMPs and is regulated by CiaRH in biofilm cells.