Transcription factor E93 regulates wing development by directly promoting Dpp signaling in Drosophila
Transcription factor E93 regulates wing development by directly promoting Dpp signaling in Drosophila
复制标题
果蝇中转录因子 E93 通过直接促进 Dpp 信号传导来调节翅膀发育
DOI：
10.1016/j.bbrc.2019.03.100
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Daojun Cheng
中科院分区：
文献类型：
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作者：
Weina Wang;Jian Peng;Zheng Li;Peng Wang;Mengpei Guo;Tianlei Zhang;Wenliang Qian;Qingyou Xia;Daojun Cheng
转录因子e93是一种类固醇激素蜕皮激素早期反应基因，在昆虫预蛹-蛹-成虫的变态过程中，对幼虫组织的降解和成虫器官的形成都起着至关重要的作用。然而，e93调控的分子机制却知之甚少。在这项研究中，我们发现果蝇E93基因的特异性敲除扰乱了翅膀的发育。对果蝇e93基因的芯片序列分析表明，DPP基因是e93的一个潜在下游靶点。芯片-聚合酶链式反应和双荧光素酶报告基因分析证实，e93能与DpP启动子结合并增强其活性。此外，DPP信号通路中DPP等成分的表达随着E93在果蝇S2细胞中的过度表达而上调，但在E93在翅膀中被敲除后表达降低。此外，DPP信号通路的损伤也反映了E93基因敲除对翅膀发育的影响。综上所述，我们的结果表明，e93在果蝇变态过程中调节DPP信号通路来调节翅膀的发育。
Transcription factor E93 is a steroid hormone ecdysone early response gene and plays crucial roles in both the degradation of larval tissues and the formation of adult organs during insect metamorphosis with the prepupal-pupal-adult transition. However, the molecular mechanism underlying E93 regulation is poorly understood. In this study, we found that specific knockdown of theE93gene in theDrosophilawing disrupted wing development. Analyzing ChIP-seq signals for E93 inDrosophilawing identified that the decapentaplegic (Dpp) gene was a potential downstream target of E93. ChIP-PCR analysis and dual-luciferase reporter assay confirmed that E93 could bind to theDpppromoter and enhanced its activity. Furthermore, the expressions ofDppand other components in the Dpp signaling pathway were upregulated followingE93overexpression inDrosophilaS2 cells but were decreased afterE93knockdown in the wing. Moreover, the impairment of the Dpp signaling pathway phenocopied the defects ofE93knockdown on wing development. Taken together, our results suggest that E93 modulates the Dpp signaling pathway to regulate wing development duringDrosophilametamorphosis.