Chitosan oligosaccharide induces resistance to Pst DC3000 in Arabidopsis via a non-canonical N-glycosylation regulation pattern
Chitosan oligosaccharide induces resistance to Pst DC3000 in Arabidopsis via a non-canonical N-glycosylation regulation pattern
复制标题
壳寡糖通过非规范的 N-糖基化调控模式诱导拟南芥对 Pst DC3000 的抗性
DOI：
10.1016/j.carbpol.2020.116939
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发表时间：
2020-12-15
影响因子：
11.2
通讯作者：
Yin, Heng
中科院分区：
文献类型：
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作者：
Jia, Xiaochen;Zeng, Haihong;Yin, Heng
研究了蛋白质N-糖基化在壳寡糖诱导抗性中的作用。结果表明，N-糖基化缺陷的拟南芥突变体（stt 3a和ManI）对假单胞菌的敏感性更高。番茄DC 3000（Pst DC 3000）的生长速率比野生型（WT）植物的生长速率高。令人惊讶的是，在stt 3a和Mani中，COS诱导的对Pst DC 3000的抗性大部分是完整的，并且对SA和JA介导的信号传导途径的上调作用也与WT相似。COS处理后，核苷酸糖积累和N-糖基化相关基因的表达受到不同程度的调控。全局糖组学分析定量了157种N-聚糖异构体，并且它们中的56.7%、50.3%和47.1%分别在COS、模拟+ Pst和COS + Pst处理的植物中显著改变。此外，COS预处理可以逆转Pst DC 3000对许多N-聚糖的影响，表明COS通过非典型模式调控蛋白质的N-糖基化，这可能是COS在N-糖基化受损时具有明显的防病效果的原因。
Roles of protein N-glycosylation in chitosan oligosaccharide (COS) induced resistance were investigated in the present study. Results demonstrated that N-glycosylation deficient Arabidopsis mutants (stt3a and ManI) were more susceptible against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) than wild type (WT) plants. Surprisingly, in stt3a and Mani, COS-induced resistance to Pst DC3000 was mostly intact, and the up-regulation effect on SA- and JA-mediated signalling pathways also similar like WT. Nucleotide sugars accumulation and N-glycosylation related genes expression were differently regulated after COS treatment. Global glycomics analysis quantified 157 N-glycan isomers, and 56.7, 50.3 and 47.1 % of them were significantly changed in COS, mock + Pst, and COS + Pst treated plants, respectively. Moreover, COS pretreatment could reverse the effect of Pst DC3000 on many N-glycans, suggesting that COS regulates protein N-glycosylation via a non-canonical pattern compared with plant defense, which may contribute to its obvious disease control effect when N-glycosylation impairment occurs.