Human papillomavirus gene expression in cutaneous squamous cell carcinomas from immunosuppressed and immunocompetent individuals.
Human papillomavirus gene expression in cutaneous squamous cell carcinomas from immunosuppressed and immunocompetent individuals.
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DOI：
10.1111/j.0022-202x.2005.23635.x
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发表时间：
2005-07
期刊：
影响因子：
--
通讯作者：
Breuer J
中科院分区：
文献类型：
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作者：
Purdie KJ;Surentheran T;Sterling JC;Bell L;McGregor JM;Proby CM;Harwood CA;Breuer J
通过PCR方法在器官移植受者（OTR）和免疫活性（IC）个体的鳞状细胞癌（SCC）中检测到疣状表皮发育不良（EV）型人乳头瘤病毒（HPV）DNA。它们在皮肤癌发展中的作用尚不清楚，以前的研究也没有解决这些病毒是否具有转录活性。在这里，我们使用原位杂交（ISH）来研究这些病毒的转录活性和DNA定位。PCR阳性的11例OTR SCC中4例（36%）、2例IC SCC中1例（50%）和5例OTR疣中1例（20%）检测到EV-HPV基因转录本。当检测到病毒DNA时，病毒DNA与E2/E4早期区域基因转录物共定位于中表皮层或上表皮层。非EV皮肤HPV基因转录在1/5（20%）OTR SCC和4/10（40%）OTR疣中被证实。在混合感染存在PCR，转录为两种类型的6例（33%）中检测到2。我们的研究结果提供了证据EV-HPV基因表达SCC。虽然只有一部分肿瘤呈阳性，但病毒疣中类似的低转录活性表明这可能是低估了。这些观察结果，以及新出现的流行病学和功能数据，提供了进一步的理由，集中在EV-HPV类型的皮肤SCC的发病机制的贡献。
Epidermodysplasia verruciformis (EV) type human papillomavirus (HPV) DNAs have been detected by PCR in squamous cell carcinomas (SCCs) from both organ transplant recipients (OTR) and immunocompetent (IC) individuals. Their role in the development of skin cancer remains unclear, and previous studies have not addressed whether the viruses are transcriptionally active. Here we have used in situ hybridisation (ISH) to investigate the transcriptional activity and DNA localisation of these viruses. EV-HPV gene transcripts were demonstrated in 4 of 11 (36%) OTR SCC, 1 of 2 (50%) IC SCC and 1 of 5 (20%) OTR warts positive by PCR. Where detected, viral DNAs co-localised with E2/E4 early region gene transcripts in the middle or upper epidermal layers. Non-EV cutaneous HPV gene transcripts were demonstrated in 1 of 5 (20%) OTR SCC and 4 of 10 (40%) OTR warts. Where mixed infection was present by PCR, transcripts for both types were detected in 2 of 6 (33%) cases. Our results provide evidence of EV-HPV gene expression in SCCs. Although only a proportion of tumours were positive, the similarly low transcriptional activity in viral warts suggests that this may be an underestimate. These observations, together with emerging epidemiological and functional data, provide further reason to focus on the contribution of EV-HPV types to the pathogenesis of cutaneous SCC.