Glutamine Deprivation Causes Enhanced Plating Efficiency of a Herpes Simplex Virus Type 1 ICP0-Null Mutant
Glutamine Deprivation Causes Enhanced Plating Efficiency of a Herpes Simplex Virus Type 1 ICP0-Null Mutant
复制标题
DOI：
10.1128/jvi.01086-08
复制
发表时间：
2008-11-15
影响因子：
5.4
通讯作者：
Schaffer, Priscilla A.
中科院分区：
文献类型：
--
作者：
Bringhurst, Ryan M.;Dominguez, Antonia A.;Schaffer, Priscilla A.
据报道，在感染前剥夺细胞单层异亮氨酸会导致单纯疱疹病毒1型(HSV-1)ICP0缺失(ICP0(-))突变体的部分互补。我们现在报道，单独剥夺谷氨酰胺能够提高ICP0(-)病毒的培养效率，而剥夺异亮氨酸几乎没有影响。因为低水平的谷氨酰胺与应激有关，而且众所周知，应激可以诱导重新激活，因此低水平的谷氨酰胺可能与HSV-1从潜伏期重新激活有关。此外，我们还证明了精氨酸和蛋氨酸的缺乏会导致ICP0(-)病毒的部分互补。
Isoleucine deprivation of cellular monolayers prior to infection has been reported to result in partial complementation of a herpes simplex virus type 1 (HSV-1) ICP0 null (ICP0(-)) mutant. We now report that glutamine deprivation alone is able to enhance the plating efficiency of an ICP0(-) virus and that isoleucine deprivation has little or no effect. Because a low glutamine level is associated with stress and because stress is known to induce reactivation, low levels of glutamine may be relevant to the reactivation of HSV-1 from latency. Additionally, we demonstrate that arginine and methionine deprivation result in partial complementation of the ICP0(-) virus.