RELATIONSHIP BETWEEN THE DEVELOPMENTAL PROGRAMS CONTROLLING NUCLEAR AND CYTOPLASMIC MATURATION OF MOUSE OOCYTES
RELATIONSHIP BETWEEN THE DEVELOPMENTAL PROGRAMS CONTROLLING NUCLEAR AND CYTOPLASMIC MATURATION OF MOUSE OOCYTES
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DOI：
10.1006/dbio.1994.1175
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发表时间：
1994-07-01
影响因子：
2.7
通讯作者：
CHESNEL, F
中科院分区：
文献类型：
--
作者：
EPPIG, JJ;SCHULTZ, RM;CHESNEL, F
本文重点研究调节小鼠卵母细胞核和细胞质成熟的发育程序之间的关系。当卵母细胞接近完成其生长期时，当从小窦卵泡中分离并培养时，它们能够恢复减数分裂，但许多这些卵母细胞的减数分裂进程在中期 I (MI) 停止。然而，随着生发囊泡 (GV) 阶段的进一步生长和发育，这些卵母细胞有能力完成核成熟并进展到中期 II (MII)。在这项研究中，从 18 日龄小鼠的小窦卵泡中分离并培养 16 至 17 小时后，约 44% 的卵母细胞在 MI 时被捕获。受精后，这些 MI 停滞的卵母细胞产生第一个极体，形成原核，分裂并发育至囊胚阶段。百分之七十五的囊胚是三倍体，其余的是二倍体。用钙离子载体处理 MI 停滞的卵母细胞导致第一次减数分裂和孤雌生殖激活的完成。此外，虽然通过[S-35]蛋氨酸标记和二维凝胶电泳测定，MI 停滞卵母细胞中的蛋白质合成模式与正常 MI 卵母细胞有很大不同，但与 MII 停滞卵母细胞相似。因此得出的结论是，细胞质成熟的一些关键方面可能发生在核成熟在 MI 时停滞的卵母细胞中。此外，促进 MII 停滞的卵母细胞进入后期 II 的触发因素足以驱动 MI 停滞的卵母细胞进入后期 I，并在成熟细胞质中产生触发因素时产生第一个极体。比较了从 18 日龄或 26 日龄小鼠分离后体外成熟的 MII 卵母细胞的发育能力。两组的受精和卵裂至双细胞阶段的频率相同。令人惊讶的是，当卵母细胞来自 26 日龄小鼠时，完成 2 细胞阶段到囊胚转变的能力比 18 日龄小鼠更频繁（分别为 82% 和 27%）。因此，尽管两组卵母细胞均已完成核成熟并进展至 MII，但来自年轻小鼠较小卵泡的卵母细胞仍缺乏 2 细胞阶段以上胚胎发育所必需的母体因子。因此，需要进一步分化这些小窦卵泡的 GV 期卵母细胞，以产生能够完成植入前发育的卵子。 (C) 1994 年学术出版社
This paper focuses on the relationship between the developmental programs that regulate nuclear and cytoplasmic maturation of mouse oocytes. As oocytes near completion of their growth phase, they become competent to resume meiosis when isolated from the small antral follicles and cultured, but the progression of meiosis in many of these oocytes stops at metaphase I (MI). With further growth and development at the germinal vesicle (GV) stage, however, these oocytes become competent to complete nuclear maturation and progress to metaphase II (MII). In this study, about 44% of the oocytes were arrested at MI after isolation from the small antral follicles of 18-day-old mice and culture for 16 to 17 hr. Upon insemination, these MI-arrested oocytes produced the first polar body, formed pronuclei, cleaved, and developed to the blastocyst stage. Seventy five percent of these blastocysts were triploid, the remainder were diploid. Treatment of MI-arrested oocytes with calcium ionophore resulted in the completion of the first meiotic division and parthenogenetic activation. Moreover, while the pattern of proteins synthesized in MI-arrested oocytes was quite different from that of normal MI oocytes as determined by [S-35]methionine labeling and two-dimensional gel electrophoresis, it was similar to that of MII-arrested oocytes. It was therefore concluded that some critical aspects of cytoplasmic maturation can occur in oocytes whose nuclear maturation is arrested at MI. In addition, triggers that promote entry of MII-arrested oocytes into anaphase II are sufficient to drive MI-arrested oocytes into anaphase I and to produce the first polar body when the triggers are generated in mature cytoplasm. The developmental capacity of MII oocytes that matured in vitro after isolation from 18- or 26-day-old mice were compared. The frequency of fertilization and cleavage to the 2-cell stage was equal in both groups. In surprising contrast, the ability to complete the 2-cell stage to blastocyst transition occurred much more frequently when the oocytes were from the 26- than the 18-day-old mice (82 and 27%, respectively). Thus, even though both groups of oocytes had completed nuclear maturation by progressing to MII, oocytes from the smaller follicles of the younger mice were deficient in maternal factors essential for development of embryos beyond the 2-cell stage. Further differentiation of these GV-stage oocytes of small antral follicles is therefore required to produce eggs competent of completing preimplantation development. (C) 1994 Academic Press, Inc.