The bovine salivary proteins BSP30a and BSP30b are independently expressed BPI-like proteins with anti-Pseudomonas activity
The bovine salivary proteins BSP30a and BSP30b are independently expressed BPI-like proteins with anti-Pseudomonas activity
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DOI：
10.1016/j.molimm.2007.10.032
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发表时间：
2008-04-01
影响因子：
3.6
通讯作者：
Wheeler, T.
中科院分区：
文献类型：
--
作者：
Haigh, B.;Hood, K.;Wheeler, T.
BPI样蛋白家族被认为在气道和口腔的先天免疫中起作用。它们与哺乳动物中重要的宿主防御分子杀菌/通透性增加蛋白（BPI）在其mRNA的核苷酸序列、外显子的组织及其预测的蛋白质结构方面具有相似性。我们比较了13种已知的牛BPI样蛋白中的2种BSP 30 a和B的表达和功能，它们共同构成了牛唾液中总蛋白的30%。尽管他们最近的分歧，我们发现，这两种蛋白质有独特的表达模式，相当大的动物间和动物内的丰度变化，并在其糖基化状态不同。重组和天然BSP 30 a和B对铜绿假单胞菌和肺炎链球菌表现出生长抑制活性。天然BSP 30 a和B没有显著的脂多糖结合活性。这些数据提供了支持BPI样蛋白在宿主防御中的作用的功能证据，并表明BSP 30 a和B通过独立于LPS调理作用的机制促进牛唾液的生长抑制功能。(C)2007爱思唯尔有限公司保留所有权利。
The family of BPI-like proteins are thought to play a role in innate immunity of the airways and oral cavity. They have similarities with bactericidal/permeability-increasing protein (BPI), an important host defence molecule in mammals, in the nucleotide sequence of their mRNAs, organisation of exons and their predicted protein structure. We compared the expression and function of 2 of the 13 known bovine BPI-like proteins, BSP30a and b, which together constitute 30% of the total protein in bovine saliva. Despite their recent divergence, we found that the two proteins have unique expression patterns, considerable inter- and intra-animal variation in abundance and differ in their glycosylation status. Recombinant and native BSP30a and b exhibited growth suppression activity against Pseudomonas aeruginosa and Streptococcus pneumoniae. Native BSP30a and b had no significant lipopolysaccharide-binding activity. These data provide functional evidence supporting a role for the BPI-like proteins in host defence and suggest that BSP30a and b contribute to the growth suppression function of bovine saliva through a mechanism independent of LPS opsonisation. (C) 2007 Elsevier Ltd. All rights reserved.