Visual chiral recognition of D/L-leucine using cube-shaped gold nanoparticles as colorimetric probes
Visual chiral recognition of D/L-leucine using cube-shaped gold nanoparticles as colorimetric probes
复制标题
使用立方体金纳米颗粒作为比色探针对 D/L-亮氨酸进行视觉手性识别
DOI：
10.1016/j.saa.2019.117263
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Li Baoxin
中科院分区：
文献类型：
--
作者：
Zhou Xiaojuan;Xu Chunli;Jin Yan;Li Baoxin
对映体的手性识别至关重要。在这项工作中，构建了一种有效且简单的手性传感策略来区分亮氨酸（Leu）对映体。在这种手性传感中，立方体金纳米粒子（AuNC）作为比色探针用于识别亮氨酸对映体。 L-Leu可以快速诱导AuNCs聚集，导致AuNCs溶液由红色变为无色，而D-Leu不能诱导颜色变化。这种明显的颜色变化使肉眼能够区分亮氨酸的手性异构体。应用手性传感来测量整个范围（-100% 至 100%）内 L-Leu 的对映体过量。这种手性分析可以通过肉眼或简单的紫外-可见光谱仪进行。此外，还利用圆二色性（CD）光谱、紫外可见吸收光谱和zeta电位研究了手性识别机制。在该方法中，手性识别是由于AuNCs固有的手性，并且AuNCs不需要任何手性标记或修饰。这种手性传感方法简单、廉价、快速且易于操作。此外，这项工作为纳米粒子的内在手性提供了实验证据，有助于人们理解纳米结构的手性。
Chiral recognition of enantiomers is fundamentally important. In this work, an effective and simple chiral sensing strategy for discrimination of leucine (Leu) enantiomers was constructed. In this chiral sensing, cube-shaped gold nanoparticles (AuNCs) as colorimetric probes are used to recognize Leu enantiomers. L-Leu can induce rapidly the aggregation of AuNCs, leading to change of AuNCs solution from red to colorless, while D-Leu cannot induce the color change. This distinct color changes allow naked-eye to distinguish chiral isomers of Leu. The chiral sensing was applied to measure the enantiometric excess of L-Leu in the whole range (from −100% to 100%). This chiral analysis can be performed by naked eye or simple ultraviolet-visible spectrometer. In addition, the mechanism of chiral recognition has been studied with circular dichroism (CD) spectra, UV–vis absorption spectra and zeta potential. In this method, the chiral recognition is due to the intrinsic chirality of AuNCs, and the AuNCs don't need any chiral labeling or modification. This chiral sensing method is simple, cheap, rapid and easy to operate. Furthermore, this work provides one experimental evidence for intrinsic chirality of nanoparticles, and helps people understand the chirality of nanostructures.