FRACTIONATION AND ANTIGENIC CHARACTERIZATION OF ORGANELLES OF EIMERIA-TENELLA SPOROZOITES
FRACTIONATION AND ANTIGENIC CHARACTERIZATION OF ORGANELLES OF EIMERIA-TENELLA SPOROZOITES
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DOI：
10.1017/s003118200006073x
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发表时间：
1992-02-01
期刊：
影响因子：
2.4
通讯作者：
FRAZIER, JA
中科院分区：
文献类型：
--
作者：
KAWAZOE, U;TOMLEY, FM;FRAZIER, JA
用超声破碎柔嫩艾美耳球虫子孢子，蔗糖梯度离心分离亚细胞组分。从梯度的馏分，其特征在于电子显微镜的外观和它们的多肽和抗原谱确定通过PAGE和Western印迹与抗血清子孢子和第1和第2代裂殖子。含有微线，棒状体或膜的馏分表现出显着不同的多肽含量和抗原反应性。裂殖子和第二代裂殖子之间的表位高度保守，而大多数棒状体表位和许多膜表位是子孢子特异性的。第一代裂殖子产生的抗血清强烈识别的子孢子的唯一多肽是分子量约为100 kDa的微线体抗原。
Sporozoites of Eimeria tenella were disrupted by sonication and subcellular fractions were separated by sucrose gradient ultracentrifugation. Fractions from gradients were characterized by electron microscopical appearance and their polypeptide and antigenic profiles determined by PAGE and Western blotting with antisera to sporozoites and 1st- and 2nd-generation merozoites. Fractions containing micronemes, rhoptries or membranes showed markedly different polypeptide content and antigenic reactivity. Microneme epitopes were strongly conserved between sporozoites and 2nd-generation merozoites whereas the majority of rhoptry epitopes and many membrane epitopes were sporozoite specific. The only polypeptide of sporozoites which was strongly recognized by antisera raised to 1st generation merozoites was a microneme antigen of molecular weight approximately 100 kDa.